Analytical Data
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基因名
UBE3A
- Application
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别名
UBE3A；E6AP；EPVE6AP；HPVE6A；Ubiquitin-Protein ligase E3A
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q05086-2
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表达区间
2-852aa
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氨基酸序列
KRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALE LYKINAKLCDPHPSKKGASSAYLENSKGAPNNSCSEIKMNKKGARIDFKD VTYLTEEKVYEILELCREREDYSPLIRVIGRVFSSAEALVQSFRKVKQHT KEELKSLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGDNNL QKLGPDDVSVDIDAIRRVYTRLLSNEKIETAFLNALVYLSPNVECDLTYH NVYSRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKL IRLWSKYNADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKC LKMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPR VDPLETELGVKTLDCRKPLIPFEEFINEPLNEVLEMDKDYTFFKVETENK FSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLR LKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEF FQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGLAIY NNCILDVHFPMVVYRKLMGKKGTFRDLGDSHPVLYQSLKDLLEYEGNVED DMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNK SVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETT EYDGGYTRDSVLIREFWEIVHSFTDEQKRLFLQFTTGTDRAPVGGLGKLK MIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAITYAKGFGM L
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分子量
100 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
UBE3A is an E3 ubiquitin ligase that plays a crucial role in the ubiquitin-proteasome system, which regulates protein degradation and involves various cellular processes, including cell cycle progression, DNA repair, and synaptic plasticity. Mutations or deletions in the UBE3A gene are linked to several neurodevelopmental disorders, most notably Angelman syndrome, characterized by severe intellectual disability, speech impairment, and ataxia. Understanding the function and regulatory mechanisms of UBE3A is pivotal, as it is primarily expressed from the paternal allele in neurons due to a phenomenon known as genomic imprinting, leading to challenges in therapeutic strategies. Recent research has focused on developing recombinant UBE3A proteins to investigate their structure-function relationships, regulatory pathways, and potential as therapeutic agents. Such studies aim to explore UBE3A's role in neuronal function and its implications in synaptic health, with the hope of identifying novel intervention strategies for Angelman syndrome and other related conditions. By generating and characterizing UBE3A recombinant proteins, researchers can better elucidate the molecular underpinnings of UBE3A-related disorders and potentially develop targeted treatments that could restore normal protein function or compensate for its absence in neurological contexts.
