Analytical Data
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基因名
PKR
- Application
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别名
PKR；GPR73L1；PKR2；Prokineticin receptor 2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P30613
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表达区间
1-574aa
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氨基酸序列
MSIQENISSLQLRSWVSKSQRDLAKSILIGAPGGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS
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分子量
63.8kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of PKR (Protein Kinase R) recombinant proteins has gained significant attention due to their critical role in the cellular response to viral infections and stress. PKR is an integral part of the innate immune system, functioning as a pattern recognition receptor that detects viral RNA and activates antiviral responses. When activated by double-stranded RNA, PKR phosphorylates the eukaryotic initiation factor 2 alpha (eIF2α), leading to the inhibition of protein synthesis and promoting apoptosis in infected cells. This mechanism not only limits viral replication but also enhances the expression of genes involved in the immune response. The recombinant expression of PKR allows for in-depth studies on its structure, function, and interactions with other cellular proteins, paving the way for potential therapeutic applications in viral diseases and cancer. Furthermore, understanding PKR's signaling pathways may reveal novel strategies for modulating immune responses, providing insights for vaccine development and antiviral therapies. Recent advances in biotechnology have facilitated the production of recombinant PKR, enabling researchers to explore its functional domains, regulatory mechanisms, and the impact of post-translational modifications. Thus, studying PKR recombinant proteins is pivotal for elucidating the molecular underpinnings of immune regulation and developing new treatment modalities for viral infections and other diseases linked to dysregulated PKR activity.
