Analytical Data
-
Gene name
EPG5
- Application
-
Alternative Names
Ectopic P granules autophagy protein 5 homolog (C. elegans); Ectopic P granules protein 5 homolog; Epg5; EPG5_HUMAN; HEEW1; hEPG5; KIAA1632
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q9HCE0
-
Expression Region
1-464aa
-
AA Sequence
MIVCLLFMMILLAKEVQLVDQTDSPLLSLLGQTSSLSWHLVDIVSYQSVLSYFSSHYPPSIILAKESYAELIMKLLKVSAGLSIPTDSQKHLDAVPKCQAFTHQMVQFLSTLEQNGKITLAVLEQEMSKLLDDIIVFNPPDMDSQTRHMALSSLFMEVLMMMNNATIPTAEFLRGSIRTWIGQKMHGLVVLPLLTAACQSLASVRHMAETTEACITAYFKESPLNQNSGWGPILVSLQVPELTMEEFLQECLTLGSYLTLYVYLLQCLNSEQTLRNEMKVLLILSKWLEQVYPSSVEEEAKLFLWWHQVLQLSLIQTEQNDSVLTESVIRILLLVQSRQNLVAEERLSSGILGAIGFGRKSPLSNRFRVVARSMAAFLSVQVPMEDQIRLRPGSELHLTPKAQQALNALESMASSKQYVEYQDQILQATQFIRHPGHCLQDGKSFLALLVNCLYPEVHYLDHIR
-
Molecular Weight
78.8 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EPG5, also known as EPG5 homolog, plays a crucial role in the process of autophagy, particularly in the maturation of autophagosomes. This protein is part of the evolutionary conserved EPG5 family and is essential for maintaining cellular homeostasis by facilitating the degradation of misfolded proteins and damaged organelles. Research has revealed that EPG5 interacts with various autophagy-related proteins, indicating its central role in the autophagic pathway. Dysregulation of EPG5 expression has been linked to several diseases, including neurodegenerative disorders and cancer, making it a significant focus of study in cellular biology and therapeutic development. Understanding the molecular mechanisms underlying EPG5 function and its interactions with other cellular components is vital for elucidating the complexities of autophagy and its implications in human diseases. As a recombinant protein, EPG5 can be produced using different expression systems, allowing for detailed biochemical and structural studies to further comprehend its role in cellular metabolism and potential as a drug target. This research aims to provide insights into how EPG5 contributes to autophagy regulation and could pave the way for novel therapeutic strategies in conditions where autophagy is disrupted.
