Analytical Data
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Gene name
ZIC3
- Application
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Alternative Names
Heterotaxy 1; HTX; HTX1; VACTERLX; ZIC 3; Zic family member 3 (odd paired Drosophila homolog heterotaxy 1); Zic family member 3; Zic3; ZIC3_HUMAN; Zinc finger Protein 203; Zinc finger Protein of the cerebellum 3; Zinc finger Protein ZIC 3 (Zinc finger Protein of the cerebellum 3); Zinc finger Protein ZIC 3; ZNF203
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60481
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Expression Region
1-467 aa
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AA Sequence
MTMLLDGGPQFPGLGVGSFGAPRHHEMPNREPAGMGLNPFGDSTHAAAAAAAAAAFKLSPAAAHDLSSGQSSAFTPQGSGYANALGHHHHHHHHHHHTSQVPSYGGAASAAFNSTREFLFRQRSSGLSEAASGGGQHGLFAGSASSLHAPAGIPEPPSYLLFPGLHEQGAGHPSPTGHVDNNQVHLGLRGELFGRADPYRPVASPRTDPYAAGAQFPNYSPMNMNMGVNVAAHHGPGAFFRYMRQPIKQELSCKWIDEAQLSRPKKSCDRTFSTMHELVTHVTMEHVGGPEQNNHVCYWEECPREGKSFKAKYKLVNHIRVHTGEKPFPCPFPGCGKIFARSENLKIHKRTHTGEKPFKCEFEGCDRRFANSSDRKKHMHVHTSDKPYICKVCDKSYTHPSSLRKHMKVHESQGSDSSPAASSGYESSTPPAIASANSKDTTKTPSAVQTSTSHNPGLPPNFNEWYV
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Molecular Weight
77 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ZIC3, a member of the Zic family of transcription factors, is crucial for embryonic development and is primarily expressed in the central nervous system and heart. Mutations in the ZIC3 gene are associated with X-linked holoprosencephaly and various congenital heart defects, emphasizing its significance in early embryogenesis. The study of ZIC3 recombinant proteins is essential for understanding its molecular mechanisms and functional properties. By producing ZIC3 in a recombinant form, researchers can investigate its role in gene regulation, protein interactions, and its influence on cell differentiation. This has implications for uncovering the pathways that lead to developmental disorders and offers potential for therapeutic interventions. Advances in recombinant protein technology allow for the purification and characterization of ZIC3, facilitating studies on its DNA binding affinity and the identification of target genes. Moreover, examining the structure and functional domains of ZIC3 can provide insights into its regulatory mechanisms and the impact of specific mutations on its function. Thus, the research on ZIC3 recombinant protein not only enhances our understanding of developmental biology but also paves the way for future studies aimed at developing targeted treatments for congenital conditions linked to ZIC3 dysregulation.
