Analytical Data
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Gene name
E2F4
- Application
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Alternative Names
E2F 4; E2F transcription factor 4; E2F transcription factor 4 p107/p130 binding; E2F-4; E2F4; E2F4_HUMAN; p107/p130 binding protein ; Transcription factor E2F 4; Transcription factor E2F4
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16254
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Expression Region
1-413aa
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AA Sequence
MAEAGPQAPPPPGTPSRHEKSLGLLTTKFVSLLQEAKDGVLDLKLAADTLAVRQKRRIYDITNVLEGIGLIEKKSKNSIQWKGVGPGCNTREIADKLIELKAEIEELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYVTHEDICRCFAGDTLLAIRAPSGTSLEVPIPEGLNGQKKYQIHLKSVSGPIEVLLVNKEAWSSPPVAVPVPPPEDLLQSPSAVSTPPPLPKPALAQSQEASRPNSPQLTPTAVPGSAEVQGMAGPAAEITVSGGPGTDSKDSGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSNSNSSSSSGPNPSTSFEPIKADPTGVLELPKELSEIFDPTRECMSSELLEELMSSEVFAPLLRLSPPPGDHDYIYNLDESEGVCDLFDVPVLNL
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Molecular Weight
70.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
E2F4 is a member of the E2F family of transcription factors, which play a crucial role in regulating gene expression and controlling the cell cycle, particularly the transition from the G1 to S phase. Research on E2F4 has gained momentum due to its dual role as a transcriptional activator and repressor, depending on its interaction with different cofactors and its cellular context. E2F4 is often associated with the retinoblastoma protein (pRb) pathway, which is essential for cell proliferation and tumor suppression. Dysregulation of E2F4 has been implicated in various cancers, highlighting its potential as a therapeutic target. Moreover, emerging evidence suggests that E2F4 may influence cellular differentiation and development beyond traditional cell cycle roles. Studies have also indicated its involvement in responses to cellular stress and DNA damage, suggesting a broader functional significance in maintaining genomic stability. Understanding the precise mechanisms of E2F4 action, its regulatory networks, and interactions with other cellular pathways is critical for elucidating its role in cancer and normal physiology, paving the way for novel therapeutic strategies in oncology. Therefore, ongoing research aims to dissect the complex regulatory roles of E2F4 in both normal and pathological conditions, while exploring its potential as a biomarker for disease progression and response to treatment.
