Analytical Data
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Gene name
TCF12
- Application
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Alternative Names
bHLHb20; Class B basic helix-loop-helix Protein 20; DNA binding Protein HTF4 ; DNA-binding Protein HTF4; E box binding Protein; E-box-binding Protein; HEB; Helix loop helix transcription factor 4; HsT17266; HTF4; HTF4_HUMAN; TCF-12; Tcf12; Transcription factor 12; Transcription factor HTF-4; Transcription factor HTF4
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99081
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Expression Region
1-682 aa
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AA Sequence
MNPQQQRMAA IGTDKELSDL LDFSAMFSPP VNSGKTRPTT LGSSQFSGSG IDERGGTTSW GTSGQPSPSY DSSRGFTDSP HYSDHLNDSR LGAHEGLSPT PFMNSNLMGK TSERGSFSLY SRDTGLPGCQ SSLLRQDLGL GSPAQLSSSG KPGTAYYSFS ATSSRRRPLH DSAALDPLQA KKVRKVPPGL PSSVYAPSPN SDDFNRESPS YPSPKPPTSM FASTFFMQDG THNSSDLWSS SNGMSQPGFG GILGTSTSHM SQSSSYGNLH SHDRLSYPPH SVSPTDINTS LPPMSSFHRG STSSSPYVAA SHTPPINGSD SILGTRGNAA GSSQTGDALG KALASIYSPD HTSSSFPSNP STPVGSPSPL TGTSQWPRPG GQAPSSPSYE NSLHSLQSRM EDRLDRLDDA IHVLRNHAVG PSTSLPAGHS DIHSLLGPSH NAPIGSLNSN YGGSSLVASS RSASMVGTHR EDSVSLNGNH SVLSSTVTTS STDLNHKTQE NYRGGLQSQS GTVVTTEIKT ENKEKDENLH EPPSSDDMKS DDESSQKDIK VSSRGRTSST NEDEDLNPEQ KIEREKERRM ANNARERLRV RDINEAFKEL GRMCQLHLKS EKPQTKLLIL HQAVAVILSL EQQVRERNLN PKAACLKRRE EEKVSAVSAE PPTTLPGTHP GLSETTNPMG HM
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Molecular Weight
72.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TCF12, also known as E2A, is a transcription factor belonging to the basic helix-loop-helix (bHLH) family, which plays a crucial role in various biological processes, including cell differentiation, proliferation, and tumorigenesis. Its involvement in the development of the immune system and neural tissues has made it a subject of intense research. Recent studies have highlighted TCF12's function in regulating gene expression during embryonic development and its potential link to cancer progression, particularly in the context of glioblastoma and other malignancies. Researchers are particularly interested in TCF12's ability to dimerize with other bHLH proteins, influencing cellular fate decisions. Recombining TCF12 protein allows for the exploration of its structural and functional properties, facilitating in-depth studies on its interactions with DNA and other transcription factors. Understanding the molecular mechanisms behind TCF12's activity could provide insights into the pathways that govern normal cellular functions and disease states, making it a critical focus in cancer biology and regenerative medicine. Thus, ongoing research into TCF12, especially in the context of recombinant protein technology, has significant implications for therapeutic strategies and the development of targeted treatments in oncology.
