Analytical Data
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Gene name
TBCD
- Application
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Alternative Names
Beta tubulin cofactor D; Beta-tubulin cofactor D; KIAA0988; SSD 1; SSD-1; SSD1; tbcd; TBCD_HUMAN; tfcD; Tubulin folding cofactor D; Tubulin specific chaperone D; Tubulin-folding cofactor D; Tubulin-specific chaperone D
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9BTW9
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Expression Region
1-623 aa
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AA Sequence
MKISHWDGVIRELAARALHNLAQQAPEFSATQVFPRLLSMTLSPDLHTRHGSILACAEVAYALYKLAAQENRPVTDHLDEQAVQGLKQIHQQLYDRQLYRGLGGQLMRQAVCVLIEKLSLSKMPFRGDTVIDGWQWLINDTLRHLHLISSHSRQQMKDAAVSALAALCSEYYMKEPGEADPAIQEELITQYLAELRNPEEMTRCGFSLALGALPGFLLKGRLQQVLTGLRAVTHTSPEDVSFAESRRDGLKAIARICQTVGVKAGAPDEAVCGENVSQIYCALLGCMDDYTTDSRGDVGTWVRKAAMTSLMDLTLLLARSQPELIEAHTCERIMCCVAQQASEKIDRFRAHAASVFLTLLHFDSPPIPHVPHRGELEKLFPRSDVASVNWSAPSQAFPRITQLLGLPTYRYHVLLGLVVSLGGLTESTIRHSTQSLFEYMKGIQSDPQALGSFSGTLLQIFEDNLLNERVSVPLLKTLDHVLTHGCFDIFTTEEDHPFAVKLLALCKKEIKNSKDIQKLLSGIAVFCGMVQFPGDVRRQALLQLCLLLCHRFPLIRKTTASQVYETLLTYSDVVGADVLDEVVTVLSDTAWDAELAVVREQRNRLCDLLGVPRPQLVPQPGAC
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Molecular Weight
95.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TBCD (Tubulin Folding Cofactor D) is a crucial protein that plays a significant role in the biogenesis of microtubules, which are essential components of the cytoskeleton in eukaryotic cells. Microtubules are involved in various cellular processes, including maintenance of cell shape, intracellular transport, and cell division. TBCD functions primarily by binding to tubulin dimers and facilitating their correct folding and assembly into microtubules. Research has shown that TBCD is essential for the stability of microtubules and their dynamic behavior, which is vital for proper cellular function. Abnormalities in TBCD expression or function can lead to various diseases, including neurodegenerative disorders and cancer, highlighting its importance in cellular health. Understanding the structure and function of TBCD through recombinant protein studies can provide insights into its mechanisms and potential therapeutic targets. The recombinant production of TBCD enables detailed biochemical and biophysical analyses, allowing researchers to elucidate its interactions with tubulin and other cofactors. Furthermore, studying TBCD’s role in microtubule dynamics and cellular processes can reveal new aspects of cytoskeletal regulation, potentially leading to novel approaches in treating diseases associated with microtubule dysfunction. Overall, TBCD represents a significant focus of research in cell biology, with implications for understanding fundamental cellular mechanisms and developing innovative therapeutic strategies.
