Analytical Data
-
Gene name
PDK3
- Application
-
Alternative Names
PDK3；PDHK3；[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3. mitochondrial
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q15120
-
Expression Region
1-406aa
-
AA Sequence
MRLFRWLLKQPVPKQIERYSRFSPSPLSIKQFLDFGRDNACEKTSYMFLRKELPVRLANTMREVNLLPDNLLNRPSVGLVQSWYMQSFLELLEYENKSPEDPQVLDNFLQVLIKVRNRHNDVVPTMAQGVIEYKEKFGFDPFISTNIQYFLDRFYTNRISFRMLINQHTLLFGGDTNPVHPKHIGSIDPTCNVADVVKDAYETAKMLCEQYYLVAPELEVEEFNAKAPDKPIQVVYVPSHLFHMLFELFKNSMRATVELYEDRKEGYPAVKTLVTLGKEDLSIKISDLGGGVPLRKIDRLFNYMYSTAPRPSLEPTRAAPLAGFGYGLPISRLYARYFQGDLKLYSMEGVGTDAVIYLKALSSESFERLPVFNKSAWRHYKTTPEADDWSNPSSEPRDASKYKAKQ
-
Molecular Weight
69 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PDK3 (Pyruvate Dehydrogenase Kinase 3) is a critical regulatory enzyme involved in the modulation of glucose metabolism and energy production within cells. It plays a significant role in the pyruvate dehydrogenase complex (PDC) regulation, which is essential for converting pyruvate to acetyl-CoA, a key substrate for the tricarboxylic acid (TCA) cycle. The dysregulation of PDK3 has been implicated in various metabolic disorders, including obesity, diabetes, and cancer, as it can inhibit aerobic respiration and promote a shift towards anaerobic glycolysis, often known as the Warburg effect in tumors. Research into recombinant PDK3 protein has garnered substantial interest as it provides insights into the enzyme's structure-function relationship and its biochemical pathways. Utilizing recombinant DNA technology, researchers can produce PDK3 in greater quantities for in vitro studies, facilitating the exploration of its enzymatic activity, inhibition mechanisms, and interactions with other metabolic regulators. Additionally, understanding the role of PDK3 in energy metabolism can reveal potential therapeutic targets for managing metabolic diseases and cancer treatment. The development of specific inhibitors or modulators of PDK3 may offer new avenues for pharmaceutical intervention, making it a focal point of ongoing research in both basic and applied biomedical sciences. As a result, PDK3 is not only a key player in metabolic regulation but also a promising target for therapeutic strategies aimed at combating metabolic diseases and cancer progression.
