Analytical Data
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基因名
WIPI1
- Application
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别名
ATG 18; ATG18; Atg18 Protein homolog; ATG18A; FLJ10055; WD repeat domain phosphoinositide interacting 1; WD repeat domain phosphoinositide interacting Protein 1; WD repeat domain phosphoinositide-interacting Protein 1; WD40 repeat Protein interacting with phosphoinositides of 49 kDa; WD40 repeat Protein interacting with phosphoInositides of 49kDa; WIPI 1 alpha; WIPI 1; WIPI 49; WIPI 49 kDa; WIPI-1; wipi1; WIPI1_HUMAN; WIPI49
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q5MNZ9
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表达区间
1-446 aa
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氨基酸序列
MEAEAADAPP GGVESALSCF SFNQDCTSLA TGTKAGYKLF SLSSVEQLDQ VHGSNEIPDV YIVERLFSSS LVVVVSHTKP RQMNVYHFKK GTEICNYSYS SNILSIRLNR QRLLVCLEES IYIHNIKDMK LLKTLLDIPA NPTGLCALSI NHSNSYLAYP GSLTSGEIVL YDGNSLKTVC TIAAHEGTLA AITFNASGSK LASASEKGTV IRVFSVPDGQ KLYEFRRGMK RYVTISSLVF SMDSQFLCAS SNTETVHIFK LEQVTNSRPE EPSTWSGYMG KMFMAATNYL PTQVSDMMHQ DRAFATARLN FSGQRNICTL STIQKLPRLL VASSSGHLYM YNLDPQDGGE CVLIKTHSLL GSGTTEENKE NDLRPSLPQS YAATVARPSA SSASTVPGYS EDGGALRGEV IPEHEFATGP VCLDDENEFP PIILCRGNQK GKTKQS
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分子量
48.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
WIPI1 (WD Repeat domain phosphoinositide-interacting protein 1) is a crucial protein involved in the process of autophagy, a cellular degradation pathway essential for maintaining cellular homeostasis and responding to stress. The importance of WIPI1 arises from its role in the formation of autophagosomes, which are necessary for sequestering damaged organelles and proteins for degradation. Research has shown that WIPI1 interacts with phosphoinositides, particularly phosphatidylinositol 3-phosphate, to facilitate the recruitment of other autophagy-related proteins, thereby promoting the autophagic process. Given its pivotal role in autophagy regulation, WIPI1 has garnered attention in studies related to various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. The recombinant expression of WIPI1 provides a valuable tool for elucidating its functional mechanisms and regulatory processes. By studying WIPI1 in a controlled environment, researchers can investigate its interactions with other proteins and its impact on autophagy under different physiological and pathological conditions. Understanding the biological functions of WIPI1 could offer insights into potential therapeutic targets for diseases where autophagy is dysregulated, making it a subject of intense research interest in cellular biology and medical sciences.
