Analytical Data
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Gene name
WDSUB1
- Application
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Alternative Names
WDSUB1; WDSAM1; WD repeat; SAM and U-box domain-containing Protein 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8N9V3
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Expression Region
1-476 aa
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AA Sequence
MVKLIHTLAD HGDDVNCCAF SFSLLATCSL DKTIRLYSLR DFTELPHSPL KFHTYAVHCC CFSPSGHILA SCSTDGTTVL WNTENGQMLA VMEQPSGSPV RVCQFSPDST CLASGAADGT VVLWNAQSYK LYRCGSVKDG SLAACAFSPN GSFFVTGSSC GDLTVWDDKM RCPHSEKAHD LGITCCDFSS QPVSDGEQGL QFFRLASCGQ DCQVKIWIVS FTHILGFELK YKSTLSGHCA PVLACAFSHD GQMLVSGSVD KSVIVYDTNT ENILHTLTQH TRYVTTCAFA PNTLLLATGS MDKTVNIWQF DLETLCQARR TEHQLKQFTE DWSEEDVSTW LCAQDLKDLV GIFKMNNIDG KELLNLTKES LADDLKIESL GLRSKVLRKI EELRTKVKSL SSGIPDEFIC PITRELMKDP VIASDGYSYE KEAMENWISK KKRTSPMTNL VLPSAVLTPN RTLKMAINRW LETHQK
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Molecular Weight
52.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
WDSUB1, or Wnt-Dickkopf signaling pathway inhibitor-1, is an important protein that plays a significant role in the regulation of Wnt signaling, which is a critical pathway implicated in various biological processes, including embryonic development, cell proliferation, and differentiation. Dysregulation of Wnt signaling has been associated with numerous diseases, particularly cancer, making the study of WDSUB1 particularly relevant in the context of therapeutic interventions. Research has indicated that WDSUB1 functions by inhibiting the activity of Wnt ligands, thereby modulating downstream signaling cascades. This protein has garnered attention for its potential as a biomarker and therapeutic target in cancers that exhibit aberrant Wnt signaling. Furthermore, the recombinant expression of WDSUB1 allows for in-depth functional studies, which can elucidate its mechanism of action and role in oncogenesis. Scientists are engaged in efforts to produce and characterize recombinant WDSUB1 to investigate its structural properties, biological functions, and interactions with other signaling molecules. Understanding the nuances of WDSUB1's interactions within the Wnt signaling pathway offers promising avenues for the development of novel therapeutic strategies aimed at restoring normal signaling and inhibiting tumor progression. As research progresses, elucidating the precise biological functions of WDSUB1 will be pivotal in advancing targeted cancer therapies and enhancing our understanding of the underlying mechanisms driving tumorigenesis.
