Cat: PAX2000-12549

Recombinant Human WDR85 Protein,His

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Analytical Data

  • Gene name

    WDR85

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    C9orf112; Diphthamide biosynthesis 7; DPH7; FLJ90634; OTTHUMP00000022705; RP11-48C7.3; RRT2; WD repeat domain 85; WD repeat-containing Protein 85; wdr85; WDR85_HUMAN

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q9BTV6

  • Expression Region

    1-452 aa

  • AA Sequence

    MMGCFALQTV DTELTADSVE WCPLQGCRHL LACGTYQLRR PEDRPAGPQN KGGMEVKEPQ VRLGRLFLYS FNDNNSIHPL VEVQRKDTSA ILDMKWCHIP VAGHALLGLA DASGSIQLLR LVESEKSHVL EPLSSLALEE QCLALSLDWS TGKTGRAGDQ PLKIISSDST GQLHLLMVNE TRPRLQKVAS WQAHQFEAWI AAFNYWHPEI VYSGGDDGLL RGWDTRVPGK FLFTSKRHTM GVCSIQSSPH REHILATGSY DEHILLWDTR NMKQPLADTP VQGGVWRIKW HPFHHHLLLA ACMHSGFKIL NCQKAMEERQ EATVLTSHTL PDSLVYGADW SWLLFRSLQR APSWSFPSNL GTKTADLKGA SELPTPCHEC REDNDGEGHA RPQSGMKPLT EGMRKNGTWL QATAATTRDC GVNPEEADSA FSLLATCSFY DHALHLWEWE GN

  • Molecular Weight

    50.5 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

WDR85, or WD repeat-containing protein 85, is a member of the WD-repeat protein family, which plays crucial roles in various cellular processes including signal transduction, cell cycle regulation, and RNA processing. Research on WDR85 has gained traction due to its potential involvement in neurodevelopmental disorders and its association with diseases like cancer. Initial studies suggest that WDR85 may interact with other proteins involved in essential cellular pathways, which could shed light on its functional significance. Investigating the recombinant form of WDR85 is pivotal for understanding its structure-function relationships and elucidating its role in cellular mechanisms. This reconstituted form can be utilized in biochemical assays, allowing researchers to explore binding properties, post-translational modifications, and potential interactions with other biomolecules. Moreover, insights gained from WDR85 recombinant protein studies have the potential to inform therapeutic strategies for conditions linked to its dysregulation. As the understanding of WDR85’s biological importance deepens, it may emerge as a promising target for drug development, making the study of its recombinant protein not only relevant but essential in current biomedical research.

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