Analytical Data
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基因名
TAF7
- Application
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别名
RNA polymerase II TBP-associated factor subunit F; TAF 7; TAF(II)55; TAF2F; TAF7; TAF7 RNA polymerase II; TAF7 RNA polymerase II TATA box binding protein (TBP) associated factor 55 kDa; TAF7_HUMAN; TAFII 55; TAFII-55; TAFII55; TATA box binding protein (TBP) associated factor RNA polymerase II F 55kD; TATA box binding protein associated factor 2F; TBP associated factor F; Transcription factor IID subunit TAFII55; Transcription initiation factor TFIID 55 kDa subunit; Transcription initiation factor TFIID subunit 7
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15545
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表达区间
1-349 aa
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氨基酸序列
MSKSKDDAPH ELESQFILRL PPEYASTVRR AVQSGHVNLK DRLTIELHPD GRHGIVRVDR VPLASKLVDL PCVMESLKTI DKKTFYKTAD ICQMLVSTVD GDLYPPVEEP VASTDPKASK KKDKDKEKKF IWNHGITLPL KNVRKRRFRK TAKKKYIESP DVEKEVKRLL STDAEAVSTR WEIIAEDETK EAENQGLDIS SPGMSGHRQG HDSLEHDELR EIFNDLSSSS EDEDETQHQD EEDINIIDTE EDLERQLQDK LNESDEQHQE NEGTNQLVMG IQKQIDNMKG KLQETQDRAK RQEDLIMKVE NLALKNRFQA VLDELKQKED REKEQLSSLQ EELESLLEK
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分子量
40.2kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TAF7, a crucial component of the transcriptional machinery, is a subunit of the TFIID complex, which is fundamental for the initiation of transcription by RNA polymerase II. It is involved in the recruitment of RNA polymerase II to the promoter regions of genes, playing a vital role in regulating gene expression. Research into TAF7 recombinant protein has gained significance due to its implications in understanding the molecular mechanisms of transcription regulation and its potential role in various diseases, including cancer and genetic disorders. The study of TAF7 can provide insights into the intricate processes governing cellular functions and the consequences of transcriptional dysregulation. By producing and analyzing recombinant TAF7 proteins, researchers aim to elucidate its structural properties, interaction with other transcription factors, and the functional consequences of its activity in the transcription cycle. This research can pave the way for therapeutic strategies targeting transcriptional regulation, highlighting the importance of TAF7 in cellular biology and disease pathology.
