Analytical Data
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基因名
GAL3ST1
- Application
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别名
GAL3ST1；CST；Galactosylceramide sulfotransferase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q99999
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表达区间
1-423aa
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氨基酸序列
MLPPQKKPWESMAKGLVLGALFTSFLLLVYSYAVPPLHAGLASTTPEAAASCSPPALEPEAVIRANGSAGECQPRRNIVFLKTHKTASSTLLNILFRFGQKHRLKFAFPNGRNDFDYPTFFARSLVQDYRPGACFNIICNHMRFHYDEVRGLVPTNAIFITVLRDPARLFESSFHYFGPVVPLTWKLSAGDKLTEFLQDPDRYYDPNGFNAHYLRNLLFFDLGYDNSLDPSSPQVQEHILEVERRFHLVLLQEYFDESLVLLKDLLCWELEDVLYFKLNARRDSPVPRLSGELYGRATAWNMLDSHLYRHFNASFWRKVEAFGRERMAREVAALRHANERMRTICIDGGHAVDAAAIQDEAMQPWQPLGTKSILGYNLKKSIGQRHAQLCRRMLTPEIQYLMDLGANLWVTKLWKFIRDFLRW
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
GAL3ST1, or Galactose-3-O-sulfotransferase 1, is an enzyme critical for the synthesis of sulfated glycosaminoglycans, particularly heparan sulfate, which plays essential roles in various biological processes, including cell signaling, regulation of growth factors, and modulation of blood coagulation. Abnormal expression or function of GAL3ST1 has been implicated in several pathologies, including cancer metastasis and developmental disorders. As such, understanding the structure and function of this enzyme is pivotal for both basic and applied research. Recent studies have focused on characterizing recombinant GAL3ST1 proteins to elucidate their biochemical properties and interactions with substrates, providing insights into its catalytic mechanisms. Techniques such as site-directed mutagenesis, crystallography, and enzymatic assays are employed to study the enzyme's activity and specificity. The production of GAL3ST1 as a recombinant protein not only aids in dissecting its functional roles in physiological and pathological contexts but also opens avenues for therapeutic interventions by developing inhibitors or substrates that target its activity. The findings from these studies may reveal novel strategies for treating diseases linked to glycosaminoglycan dysregulation and enhance our understanding of glycosylation processes in cell biology.
