Analytical Data
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Gene name
DNASE1L1
- Application
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Alternative Names
Deoxyribonuclease I-like 1; Deoxyribonuclease-1-like 1; DNAS1L1; DNase I like 1; DNase I like muscle specific; DNase I lysosomal like; DNase I-like 1; DNase X; DNASE1L1; DNASEX; DNL1L
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P49184
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Expression Region
18-302aa
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AA Sequence
AFRICAFNAQRLTLAKVAREQVMDTLVRILARCDIMVLQEVVDSSGSAIPLLLRELNRFDGSGPYSTLSSPQLGRSTYMETYVYFYRSHKTQVLSSYVYNDEDDVFAREPFVAQFSLPSNVLPSLVLVPLHTTPKAVEKELNALYDVFLEVSQHWQSKDVILLGDFNADCASLTKKRLDKLELRTEPGFHWVIADGEDTTVRASTHCTYDRVVLHGERCRSLLHTAAAFDFPTSFQLTEEEALNISDHYPVEVELKLSQAHSVQPLSLTVLLLLSLLSPQLCPAA
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Molecular Weight
57.09 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNASE1L1, a member of the DNase I family, plays a crucial role in the regulation of extracellular DNA levels and the clearance of cell debris and apoptotic cells from the bloodstream. Its significance has garnered increasing attention in the context of various diseases, particularly in autoimmune disorders and inflammatory conditions where elevated levels of extracellular DNA can contribute to pathology. Research has shown that DNASE1L1 is essential for maintaining immune homeostasis, as deficiencies in this enzyme can lead to autoimmune responses and hinder the body’s ability to resolve inflammation. The recombinant form of DNASE1L1 serves as a valuable tool in investigating its structural and functional properties, offering insights into its enzymatic activity and potential therapeutic applications. Several studies have focused on characterizing this protein's interactions with DNA and its role in modulating immune responses, highlighting its potential as a biomarker for disease progression and a target for innovative therapeutic strategies. The development of recombinant DNASE1L1 has enabled advancements in our understanding of its biological significance, paving the way for novel treatments that could harness its ability to degrade harmful extracellular DNA, thus presenting a promising avenue for addressing autoimmune and inflammatory diseases.
