Analytical Data
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基因名
VPS53
- Application
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别名
FLJ10979; FLJ41112; FLJ61757; HCCS1; hVps53L; MGC39512; pp13624; Vacuolar Protein sorting 53 (yeast); Vacuolar Protein sorting 53; Vacuolar Protein sorting 53 homolog (S. cerevisiae); Vacuolar Protein sorting-associated Protein 53 homolog; VPS53; VPS53_HUMAN
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q5VIR6
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表达区间
1-699 aa
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氨基酸序列
MMEEEELEFV EELEAVLQLT PEVQLAIEQV FPSQDPLDRA DFNAVEYINT LFPTEQSLAN IDEVVNKIRL KIRRLDDNIR TVVRGQTNVG QDGRQALEEA QKAIQQLFGK IKDIKDKAEK SEQMVKEITR DIKQLDHAKR HLTTSITTLN HLHMLAGGVD SLEAMTRRRQ YGEVANLLQG VMNVLEHFHK YMGIPQIRQL SERVKAAQTE LGQQILADFE EAFPSQGTKR PGGPSNVLRD ACLVANILDP RIKQEIIKKF IKQHLSEYLV LFQENQDVAW LDKIDRRYAW IKRQLVDYEE KYGRMFPREW CMAERIAVEF CHVTRAELAK IMRTRAKEIE VKLLLFAIQR TTNFEGFLAK RFSGCTLTDG TLKKLESPPP STNPFLEDEP TPEMEELATE KGDLDQPKKP KAPDNPFHGI VSKCFEPHLY VYIESQDKNL GELIDRFVAD FKAQGPPKPN TDEGGAVLPS CADLFVYYKK CMVQCSQLST GEPMIALTTI FQKYLREYAW KILSGNLPKT TTSSGGLTIS SLLKEKEGSE VAKFTLEELC LICNILSTAE YCLATTQQLE EKLKEKVDVS LIERINLTGE MDTFSTVISS SIQLLVQDLD AACDPALTAM SKMQWQNVEH VGDQSPYVTS VILHIKQNVP IIRDNLASTR KYFTQFCVKF ANSFIPKFIT HLFKCKPISM VGAEQVRWT
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分子量
94.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
VPS53, also known as vacuolar protein sorting-associated protein 53, plays a crucial role in the intracellular trafficking and sorting of proteins within the endosomal and Golgi networks. Its involvement in the retrograde transport of proteins to the trans-Golgi network and the maintenance of lysosomal function underscores its significance in cellular homeostasis. Dysregulation or mutations in VPS53 have been implicated in various pathologies, including neurodegenerative diseases and metabolic disorders, which highlights its potential as a therapeutic target. Recent studies have focused on the structural characterization and functional assays of VPS53, revealing insights into its mechanism of action in the context of vesicular transport. Researchers are particularly interested in elucidating the protein-protein interactions that govern its role in cellular sorting pathways, as well as exploring its interaction with other components of the endosomal system. The development of recombinant VPS53 proteins has facilitated these investigations, providing a platform for examining the molecular dynamics underlying its function. Understanding VPS53's role at the molecular level could ultimately contribute to the design of strategies aimed at ameliorating diseases linked to its dysfunction, making it a key player in the field of cell biology and therapeutic research. This fascination with VPS53 reflects a broader interest in membrane trafficking pathways and their implications for health and disease, making it a pivotal area of study in both basic and applied biomedical research.
