Analytical Data
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Gene name
VPS37D
- Application
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Alternative Names
ESCRT-I complex subunit VPS37D; MGC35352; Vacuolar Protein sorting-associated Protein 37D; VP37D_HUMAN; VPS37D; WBSCR24; Williams-Beuren syndrome chromosomal region 24 Protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q86XT2
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Expression Region
1-251 aa
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AA Sequence
MYRARAARAG PEPGSPGRFG ILSTGQLRDL LQDEPKLDRI VRLSRKFQGL QLEREACLAS NYALAKENLA LRPRLEMGRA ALAIKYQELR EVAENCADKL QRLEESMHRW SPHCALGWLQ AELEEAEQEA EEQMEQLLLG EQSLEAFLPA FQRGRALAHL RRTQAEKLQE LLRRRERSAQ PAPTSAADPP KSFPAAAVLP TGAARGPPAV PRSLPPLDSR PVPPLKGSPG CPLGPAPLLS PRPSQPEPPH R
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Molecular Weight
27.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
VPS37D, a member of the VPS37 family, plays an essential role in the endosomal sorting and trafficking pathways within cells. This protein is implicated in the formation of multi-vesicular bodies (MVBs) and the subsequent degradation of membrane proteins through the ubiquitin-proteasome system. Recent studies have highlighted the significance of VPS37D in various cellular processes, including autophagy, signaling, and cell proliferation. Its dysfunction has been associated with several diseases, including neurodegenerative disorders and cancer, where altered protein sorting may contribute to malignant transformation or cellular degeneration. The investigation of VPS37D's recombinant forms aims to elucidate its structure-function relationships and mechanistic roles in cellular homeostasis. Through a combination of biochemical assays, structural biology techniques, and cellular assays, researchers are exploring how VPS37D interacts with other proteins in the endosomal pathway and its potential as a therapeutic target. Understanding VPS37D at the molecular level could provide insights into novel treatment strategies for diseases linked to dysfunctional endosomal transport and protein degradation pathways.
