Analytical Data
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Gene name
DNAJB14
- Application
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Alternative Names
DNAJB14; UNQ9427/PRO34683; DnaJ homolog subfamily B member 14
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8TBM8
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Expression Region
1-379aa
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AA Sequence
MEGNRDEAEKCVEIAREALNAGNREKAQRFLQKAEKLYPLPSARALLEIIMKNGSTAGNSPHCRKPSGSGDQSKPNCTKDSTSGSGEGGKGYTKDQVDGVLSINKCKNYYEVLGVTKDAGDEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGNEEQACNHQNNGRFNFHRGCEADITPEDLFNIFFGGGFPSGSVHSFSNGRAGYSQQHQHRHSGHEREEERGDGGFSVFIQLMPIIVLILVSLLSQLMVSNPPYSLYPRSGTGQTIKMQTENLGVVYYVNKDFKNEYKGMLLQKVEKSVEEDYVTNIRNNCWKERQQKTDMQYAAKVYRDDRLRRKADALSMDNCKELERLTSLYKGG
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Molecular Weight
68.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNAJB14, a member of the DnaJ/Hsp40 family of heat shock proteins, plays a crucial role in cellular protein homeostasis and stress response by facilitating the proper folding of proteins and the prevention of aggregation. Its function is particularly significant in various physiological processes, including cellular stress adaptation and the modulation of protein degradation pathways. Recent studies have suggested that DNAJB14 may be involved in the pathogenesis of several neurodegenerative diseases, as its expression levels can influence the aggregation of misfolded proteins, a hallmark of these conditions. Additionally, DNAJB14 has emerged as a potential biomarker and therapeutic target for diseases characterized by protein misfolding, such as Alzheimer's and Parkinson's disease. The recombinant production of DNAJB14 allows for detailed biochemical analysis and exploration of its mechanism of action, enabling researchers to investigate its role in cellular homeostasis and its potential as a therapeutic intervention in diseases associated with protein misfolding. Understanding the functional dynamics of DNAJB14 through recombinant protein studies can provide insights into therapeutic strategies aimed at enhancing protein quality control mechanisms and mitigating the effects of proteotoxic stress in human health.
