Analytical Data
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Gene name
VPS36
- Application
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Alternative Names
C13orf9; CGI 145; EAP45; ELL associated Protein of 45 kDa; ELL-associated Protein of 45 kDa; ESCRT-II complex subunit VPS36; Vacuolar Protein sorting associated Protein 36; Vacuolar Protein-sorting-associated Protein 36; Vps36; VPS36_HUMAN
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q86VN1
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Expression Region
1-386 aa
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AA Sequence
MDRFVWTSGL LEINETLVIQ QRGVRIYDGE EKIKFDAGTL LLSTHRLIWR DQKNHECCMA ILLSQIVFIE EQAAGIGKSA KIVVHLHPAP PNKEPGPFQS SKNSYIKLSF KEHGQIEFYR RLSEEMTQRR WENMPVSQSL QTNRGPQPGR IRAVGIVGIE RKLEEKRKET DKNISEAFED LSKLMIKAKE MVELSKSIAN KIKDKQGDIT EDETIRFKSY LLSMGIANPV TRETYGSGTQ YHMQLAKQLA GILQVPLEER GGIMSLTEVY CLVNRARGME LLSPEDLVNA CKMLEALKLP LRLRVFDSGV MVIELQSHKE EEMVASALET VSEKGSLTSE EFAKLVGMSV LLAKERLLLA EKMGHLCRDD SVEGLRFYPN LFMTQS
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Molecular Weight
43.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
VPS36, a protein that belongs to the eukaryotic VPS (vacuolar protein sorting) family, plays a crucial role in intracellular trafficking and the sorting of membrane proteins. It is part of the ESCRT (endosomal sorting complex required for transport) machinery, which is essential for the biogenesis of multivesicular bodies and the degradation of membrane proteins in lysosomes. Research has shown that VPS36 is involved in various cellular processes, including autophagy, immune response, and viral entry, making it a key player in maintaining cellular homeostasis. Abnormalities in VPS36 expression or function have been linked to various diseases, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target. Recent studies have focused on elucidating the structural and functional aspects of VPS36, including its interactions with other ESCRT components and membrane proteins, aiming to uncover the detailed mechanisms by which it contributes to cellular processes. Understanding the role of VPS36 in cellular dynamics not only provides insights into fundamental biological mechanisms but also paves the way for novel strategies in disease treatment and prevention.
