Cat: PAX2000-12476

Recombinant Human VPS37A Protein,His

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Analytical Data

  • Gene name

    VPS37A

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    VPS37A; HCRP1; Vacuolar Protein sorting-associated Protein 37A; hVps37A; ESCRT-I complex subunit VPS37A; Hepatocellular carcinoma-related Protein 1

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q8NEZ2

  • Expression Region

    1-397 aa

  • AA Sequence

    MSWLFPLTKS ASSSAAGSPG GLTSLQQQKQ RLIESLRNSH SSIAEIQKDV EYRLPFTINN LTININILLP PQFPQEKPVI SVYPPIRHHL MDKQGVYVTS PLVNNFTMHS DLGKIIQSLL DEFWKNPPVL APTSTAFPYL YSNPSGMSPY ASQGFPFLPP YPPQEANRSI TSLSVADTVS SSTTSHTTAK PAAPSFGVLS NLPLPIPTVD ASIPTSQNGF GYKMPDVPDA FPELSELSVS QLTDMNEQEE VLLEQFLTLP QLKQIITDKD DLVKSIEELA RKNLLLEPSL EAKRQTVLDK YELLTQMKST FEKKMQRQHE LSESCSASAL QARLKVAAHE AEEESDNIAE DFLEGKMEID DFLSSFMEKR TICHCRRAKE EKLQQAIAMH SQFHAPL

  • Molecular Weight

    44.3 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

VPS37A, a crucial component of the ESCRT (endosomal sorting complexes required for transport) machinery, plays a significant role in cellular processes such as protein trafficking, endosomal sorting, and the degradation of cell surface receptors. Recent studies have highlighted its involvement in the modulation of various signaling pathways and its implications in the pathogenesis of multiple diseases, including cancer and neurodegenerative disorders. The understanding of VPS37A's structure and function is essential for elucidating its role in cellular homeostasis and disease progression. Recombinant expression of VPS37A is imperative for functional studies, as it allows researchers to investigate the protein's interactions with other ESCRT components and assess its activity within different cellular contexts. Additionally, studying the post-translational modifications and dynamics of VPS37A can provide insights into its regulatory mechanisms and potential therapeutic targets. Overall, the research into VPS37A's recombinant protein design is a promising avenue for advancing our understanding of cellular dysfunctions and developing novel strategies for intervention in various diseases.

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