Analytical Data
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Gene name
DNAJB12
- Application
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Alternative Names
DNAJB12; DnaJ homolog subfamily B member 12
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NXW2
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Expression Region
1-375aa
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AA Sequence
MESNKDEAERCISIALKAIQSNQPDRALRFLEKAQRLYPTPRVRALIESLNQKPQTAGDQPPPTDTTHATHRKAGGTDAPSANGEAGGESTKGYTAEQVAAVKRVKQCKDYYEILGVSRGASDEDLKKAYRRLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQFGDDKSQAARHGHGHGDFHRGFEADISPEDLFNMFFGGGFPSSNVHVYSNGRMRYTYQQRQDRRDNQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSPRPSVGHIHRRVTDHLGVVYYVGDTFSEEYTGSSLKTVERNVEDDYIANLRNNCWKEKQQKEGLLYRARYFGDTDMYHRAQKMGTPSCSRLSEVQASLHG
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Molecular Weight
68.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DNAJB12 is a member of the Hsp40 (heat shock protein 40) family, which plays a crucial role in protein folding, translocation, and cellular stress responses. This protein acts as a co-chaperone for Hsp70, enhancing its ability to manage misfolded proteins and maintain proteostasis within the cell. Studies have shown that DNAJB12 is particularly involved in mitigating protein aggregation and facilitating the degradation of damaged proteins, thereby contributing to cellular homeostasis. Given its pivotal role in protein quality control, DNAJB12 has garnered interest in the context of neurodegenerative diseases, where protein misfolding and aggregation are central pathological features. Research indicates that modulating DNAJB12 expression may provide therapeutic avenues for conditions such as Alzheimer's disease and amyotrophic lateral sclerosis (ALS). Furthermore, recombinant DNAJB12 has been engineered to study its structural properties and functional mechanisms, making it a valuable tool for developing therapeutic strategies that target protein misfolding disorders. Understanding the full potential of DNAJB12 in various biological contexts could lead to novel interventions aimed at enhancing cellular resilience in the face of stress and disease. As such, research involving recombinant DNAJB12 not only deepens our comprehension of cellular stress responses but also holds promise for advancing treatments for a range of protein misfolding-related disorders.
