Analytical Data
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Gene name
VPS33B
- Application
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Alternative Names
VPS33BVacuolar Protein sorting-associated Protein 33B; hVPS33B
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H267
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Expression Region
2-617 aa
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AA Sequence
AFPHRPDAP ELPDFSMLKR LARDQLIYLL EQLPGKKDLF IEADLMSPLD RIANVSILKQ HEVDKLYKVE NKPALSSNEQ LCFLVRPRIK NMRYIASLVN ADKLAGRTRK YKVIFSPQKF YACEMVLEEE GIYGDVSCDE WAFSLLPLDV DLLSMELPEF FRDYFLEGDQ RWINTVAQAL HLLSTLYGPF PNCYGIGRCA KMAYELWRNL EEEEDGETKG RRPEIGHIFL LDRDVDFVTA LCSQVVYEGL VDDTFRIKCG SVDFGPEVTS SDKSLKVLLN AEDKVFNEIR NEHFSNVFGF LSQKARNLQA QYDRRRGMDI KQMKNFVSQE LKGLKQEHRL LSLHIGACES IMKKKTKQDF QELIKTEHAL LEGFNIREST SYIEEHIDRQ VSPIESLRLM CLLSITENGL IPKDYRSLKT QYLQSYGPEH LLTFSNLRRA GLLTEQAPGD TLTAVESKVS KLVTDKAAGK ITDAFSSLAK RSNFRAISKK LNLIPRVDGE YDLKVPRDMA YVFGGAYVPL SCRIIEQVLE RRSWQGLDEV VRLLNCSDFA FTDMTKEDKA SSESLRLILV VFLGGCTFSE ISALRFLGRE KGYRFIFLTT AVTNSARLME AMSEVKA
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Molecular Weight
70.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
VPS33B is a member of the VPS (vacuolar protein sorting) family, crucial for intracellular trafficking and organelle function. Mutations in the VPS33B gene have been associated with various diseases, particularly a rare genetic disorder known as Chylothorax with intestinal lymphangiectasia (CIL), which leads to severe lymphatic abnormalities and compromised lipid absorption. This disorder highlights the critical role of VPS33B in maintaining cellular homeostasis and signaling pathways. The protein operates by facilitating vesicular transport and fusion processes, enabling the proper delivery of proteins and lipids to cellular compartments, including lysosomes and the plasma membrane. Research into VPS33B’s structure and function has revealed significant insights into its role in the endosomal-lysosomal pathway, which is vital for cellular waste management and metabolic regulation. The understanding of VPS33B is further bolstered by the identification of its interacting partners, which aids in elucidating its mechanistic pathways. As studies advance, there is an increasing interest in developing therapeutic strategies targeting VPS33B-related dysfunctions, opening up potential avenues for treating associated disorders. Given its importance in fundamental cellular processes, VPS33B continues to be a focal point in research aimed at uncovering new aspects of vesicular trafficking and its implications in human health and disease.
