Analytical Data
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Gene name
VPS33A
- Application
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Alternative Names
VPS33AVacuolar Protein sorting-associated Protein 33A; hVPS33A
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96AX1
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Expression Region
1-596 aa
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AA Sequence
MAAHLSYGRV NLNVLREAVR RELREFLDKC AGSKAIVWDE YLTGPFGLIA QYSLLKEHEV EKMFTLKGNR LPAADVKNII FFVRPRLELM DIIAENVLSE DRRGPTRDFH ILFVPRRSLL CEQRLKDLGV LGSFIHREEY SLDLIPFDGD LLSMESEGAF KECYLEGDQT SLYHAAKGLM TLQALYGTIP QIFGKGECAR QVANMMIRMK REFTGSQNSI FPVFDNLLLL DRNVDLLTPL ATQLTYEGLI DEIYGIQNSY VKLPPEKFAP KKQGDGGKDL PTEAKKLQLN SAEELYAEIR DKNFNAVGSV LSKKAKIISA AFEERHNAKT VGEIKQFVSQ LPHMQAARGS LANHTSIAEL IKDVTTSEDF FDKLTVEQEF MSGIDTDKVN NYIEDCIAQK HSLIKVLRLV CLQSVCNSGL KQKVLDYYKR EILQTYGYEH ILTLHNLEKA GLLKPQTGGR NNYPTIRKTL RLWMDDVNEQ NPTDISYVYS GYAPLSVRLA QLLSRPGWRS IEEVLRILPG PHFEERQPLP TGLQKKRQPG ENRVTLIFFL GGVTFAEIAA LRFLSQLEDG GTEYVIATTK LMNGTSWIEA LMEKPF
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Molecular Weight
67.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The VPS33A protein plays a crucial role in the regulation of intracellular trafficking and membrane dynamics, particularly in the context of vesicle transport and fusion processes. This protein is a member of the VPS (vacuolar protein sorting) family, which is known for its involvement in transporting proteins to lysosomes and other membrane-bound organelles. Mutations or deficiencies in VPS33A have been linked to various genetic disorders, including Schimke immuno-osseous dysplasia, which is characterized by immunodeficiency, skeletal abnormalities, and growth retardation. Consequently, studying VPS33A and its associated pathways is essential for understanding the fundamental mechanisms of cellular transport and potential therapeutic targets for related diseases. Additionally, the characterization of VPS33A as a recombinant protein has gained interest in the scientific community, as it enables further investigation into its structure-function relationship and interactions with other cellular components. Investigating VPS33A's biochemical properties, interaction networks, and role in disease mechanisms can provide insights into the development of novel therapeutic strategies for lysosomal storage disorders and other conditions linked to vesicular transport dysfunction. Overall, the study of VPS33A serves as a critical platform for advancing our knowledge of cellular processes and their implications in human health.
