Analytical Data
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基因名
NSF
- Application
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别名
NSF；Vesicle-fusing ATPase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P46459
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表达区间
1-744aa
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氨基酸序列
MAGRSMQAARCPTDELSLTNCAVVNEKDFQSGQHVIVRTSPNHRYTFTLK THPSVVPGSIAFSLPQRKWAGLSIGQEIEVSLYTFDKAKQCIGTMTIEID FLQKKSIDSNPYDTDKMAAEFIQQFNNQAFSVGQQLVFSFNEKLFGLLVK DIEAMDPSILKGEPATGKRQKIEVGLVVGNSQVAFEKAENSSLNLIGKAK TKENRQSIINPDWNFEKMGIGGLDKEFSDIFRRAFASRVFPPEIVEQMGC KHVKGILLYGPPGCGKTLLARQIGKMLNAREPKVVNGPEILNKYVGESEA NIRKLFADAEEEQRRLGANSGLHIIIFDEIDAICKQRGSMAGSTGVHDTV VNQLLSKIDGVEQLNNILVIGMTNRPDLIDEALLRPGRLEVKMEIGLPDE KGRLQILHIHTARMRGHQLLSADVDIKELAVETKNFSGAELEGLVRAAQS TAMNRHIKASTKVEVDMEKAESLQVTRGDFLASLENDIKPAFGTNQEDYA SYIMNGIIKWGDPVTRVLDDGELLVQQTKNSDRTPLVSVLLEGPPHSGKT ALAAKIAEESNFPFIKICSPDKMIGFSETAKCQAMKKIFDDAYKSQLSCV VVDDIERLLDYVPIGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKD VLQEMEMLNAFSTTIHVPNIATGEQLLEALELLGNFKDKERTTIAQQVKG KKVWIGIKKLLMLIEMSLQMDPEYRVRKFLALLREEGASPLDFD
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分子量
109 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NSF (N-ethylmaleimide-sensitive factor) is a vital ATPase that plays a crucial role in intracellular membrane trafficking and fusion processes. It is primarily involved in the disassembly of SNARE (Soluble NSF Attachment Protein Receptor) complexes, which are essential for vesicle fusion during neurotransmitter release and various intracellular transport mechanisms. Research into NSF and its functions has gained momentum due to its implications in neurobiology, synaptic transmission, and potentially neurodegenerative diseases. Understanding the dynamics of NSF and its interactions with SNARE proteins could reveal insights into the fundamental mechanisms of cellular communication. Additionally, altered NSF activity has been linked with conditions such as Alzheimer's disease, where impaired synaptic function contributes to cognitive decline. As a result, NSF has emerged as a target for therapeutic interventions aimed at restoring synaptic efficacy. Recent studies have utilized advanced techniques, including cryo-electron microscopy and mass spectrometry, to elucidate the structural and functional properties of NSF, paving the way for novel strategies to manipulate its activity in disease contexts. Overall, NSF’s role in membrane trafficking makes it a critical focus in cellular biology and a promising candidate for future research in neurotherapeutics.
