Analytical Data
-
Gene name
SPEF1
- Application
-
Alternative Names
SPEF1; Sperm flagellar protein 1
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q58DA1
-
Expression Region
1-236aa
-
AA Sequence
MAGSVDEEAL HQLYLWVDNI PLSRPKRNLS RDFSDGVLVA EVIKFYFPKM VEMHNYVPAN SLQQKLSNWS HLNRKVLNKL NFSVPEDVMR KIAQCAPGVV ELVLIPLRQR LEERQRRRKQ GIGSLQELAP QDGTDYMDVG LSQKARGEGV PDPQGRGQLR EGRLPVPRPP GDSQALQSDP SFILQIAEKE QELLASQETV QVLQMKVRRL EHLLQLKNVR IEDLSRRLQQ AERKQR
-
Molecular Weight
27.1 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SPEF1 (Sperm Flagellar 1) is a gene that encodes a protein crucial for the proper development and function of sperm flagella. Abnormalities in SPEF1 have been linked to asthenozoospermia, a condition characterized by reduced sperm motility, which can significantly impact male fertility. The understanding of SPEF1’s role in spermatogenesis and flagellar structure is essential for unraveling the underlying mechanisms of sperm motility disorders. Research has shown that mutations in the SPEF1 gene can lead to structural defects in the axoneme of flagella, affecting the beating patterns necessary for sperm motility. Furthermore, SPEF1’s involvement in ciliary function suggests broader implications in other reproductive and non-reproductive cilia-related disorders. Recent studies have focused on the expression and functional analysis of SPEF1 recombinant proteins to elucidate its mechanistic pathways and interactions with other proteins involved in flagellar assembly. By investigating these pathways, researchers aim to develop potential therapeutic strategies for treating infertility linked to SPEF1 mutations. Understanding the functional aspects of SPEF1 through recombinant protein studies could also pave the way for novel approaches in regenerative medicine and fertility treatments.
