Analytical Data
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Gene name
DIA1
- Application
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Alternative Names
DIAPH1; deafness; autosomal dominant 1; DFNA1; DIA1; DIAP1; DIAP1_HUMAN; DIAPH1; Diaphanous homolog 1 (Drosophila); diaphanous homolog 1; Diaphanous related formin 1; Diaphanous-related formin-1; DRF1; FLJ25265; hDIA1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60610
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Expression Region
1-301aa
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AA Sequence
MGAQLSTLGHMVLFPVWFLYSLLMKLFQRSTPAITLESPDIKYPLRLIDREIISHDTRRFRFALPSPQHILGLPVGQHIYLSARIDGNLVVRPYTPISSDDDKGFVDLVIKVYFKDTHPKFPAGGKMSQYLESMQIGDTIEFRGPSGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIMKDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEAWDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDHVGHPTERCFVF
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Molecular Weight
58.85 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DIA1 recombinant protein research is rooted in the exploration of its critical role in cellular processes and disease mechanisms. DIA1, a member of the DExH-DExH family of RNA helicases, is known to be involved in diverse biological functions, including RNA metabolism, mRNA splicing, and the regulation of gene expression. Its dysregulation has been associated with various diseases, particularly in the context of cancer and viral infections, where it may influence tumor progression and viral replication. The production of DIA1 as a recombinant protein has been pivotal for in-depth functional studies, allowing researchers to investigate its biochemical properties, interaction partners, and effects on cellular pathways. This research is essential for understanding the molecular basis of its activities and potential implications in therapeutic contexts. Additionally, by employing advanced techniques such as X-ray crystallography and nuclear magnetic resonance (NMR), scientists aim to elucidate its structural characteristics, which could pave the way for the development of novel drugs that target DIA1-associated pathways. Ultimately, the study of DIA1 recombinant protein is not only significant for basic science but also holds promise for translational applications in medicine, providing insights that could lead to innovative strategies for disease intervention.
