Analytical Data
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Gene name
SPC24
- Application
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Alternative Names
FLJ90806; hSpc24; Kinetochore protein Spc24; SPBC24; spc24; SPC24 NDC80 kinetochore complex component; SPC24 NDC80 kinetochore complex component homolog; SPC24. NDC80 kinetochore complex component. homolog (S. cerevisiae); SPC24_HUMAN; Spindle pole body component 24 homolog (S. cerevisiae); Spindle pole body component 24 homolog
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8NBT2
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Expression Region
1-197aa
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AA Sequence
MAAFRDIEEV SQGLLSLLGA NRAEAQQRRL LGRHEQVVER LLETQDGAEK QLREILTMEK EVAQSLLNAK EQVHQGGVEL QQLEAGLQEA GEEDTRLKAS LLYLTRELEE LKEIEADLER QEKEVDEDTT VTIPSAVYVA QLYHQVSKIE WDYECEPGMV KGIHHGPSVA QPIHLDSTQL SRKFISDYLW SLVDTEW
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Molecular Weight
22.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SPC24 is a crucial component of the spindle assembly checkpoint (SAC) in eukaryotic cells, playing a vital role in ensuring accurate chromosome segregation during cell division. This highly conserved protein is part of the Ndc80 complex, which connects kinetochores to the mitotic spindle. Aberrations in SPC24 function can lead to chromosomal instability, a hallmark of many cancers. Research on SPC24 has gained momentum due to its potential as a therapeutic target; understanding its structure, interactions, and regulatory mechanisms could pave the way for novel anticancer strategies. Moreover, studies on SPC24 provide insights into the fundamental processes of mitosis and the intricate controls governing cell cycle progression. As scientists continue to explore the molecular dynamics of SPC24, the findings could contribute to the development of targeted therapies that address the challenges posed by malignancies associated with SAC dysfunction.
