Analytical Data
-
Gene name
SPATC1
- Application
-
Alternative Names
SPATC1; SPATA15; SPRN; Speriolin; Spermatogenesis and centriole-associated protein 1; Spermatogenesis-associated protein 15; Spermatogenic cell-specific Cdc20-binding protein
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q76KD6
-
Expression Region
1-591aa
-
AA Sequence
MSLLTNYEGL RHQIERLVRE NEELKKLVRL IRENHELKSA IKTQAGGLGI SGFTSGLGEA TAGLSSRQNN GVFLPPSPAV ANERVLEEVG IMALAPLAEM LTSLQPSATP GSLMSPLTGT LSTLLSGPAP TSQSSPLTSF LTSPIAGPLT GTLASSLGLP STGTLTPSSL VAGPVAMSQS SPLIAPVMGT VAVSLSSPLL SSTATPPGVS QNLLANPMSN LVLPEAPRLR LAEPLRGGPT GPQSPACVVP TATTKVPLST EPPQSTQDPE PLSMAFAGAP LQTSTPIGAM GTPAPKTAFS FNTSDTQAQP SAAQEQVVPA SVPTSPTTSP TVTVLASAPA LAPQVATSYT PSSTTHIAQG APHPPSRMHN SPTQNLPVPH CPPHNAHSPP RTSSSPASVN DSRGPRTTEP STKSMMEVER KLAHRKTSKF PENPRESKQL AWERLVGEIA FQLDRRILSS IFPERVRLYG FTVSNIPEKI IQASLNPSDH KLDEKLCQRL TQRYVSVMNR LQSLGYNGRV HPALTEQLVN AYGILRERPE LAASEGGPYT VDFLQRVVVE TVHPGMLADA LLLLSCLSQL AHDDGKPMFI W
-
Molecular Weight
62.3 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SPATC1 (Sperm Associated Antigen 1) is a protein with emerging significance in reproductive biology and developmental processes. It is primarily expressed in the male germ cells and is believed to play a crucial role in sperm function and fertilization. Studies have suggested that SPATC1 is involved in the regulation of specific cellular functions, including gene expression and cellular signaling pathways critical for spermatogenesis. Additionally, abnormalities in SPATC1 expression or function have been linked to male infertility, making it a potential biomarker for reproductive health. Given the increasing prevalence of infertility worldwide, understanding the detailed mechanisms by which SPATC1 operates can open new avenues for therapeutic interventions. This has led to growing interest in the production of recombinant SPATC1 protein for structural and functional studies, aiming to elucidate its role in sperm biology and to explore its potential as a target in male fertility treatments. The recombinant approach allows for comprehensive biochemical analysis and the assessment of SPATC1’s interaction with other proteins involved in sperm maturation and motility, further advancing our knowledge in the field.
