Analytical Data
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Gene name
Rock1
- Application
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Alternative Names
Rock1；Rho-associated Protein kinase 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13464
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Expression Region
1-535aa
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AA Sequence
MSTGDSFETRFEKMDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQL
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Rock1, or Rho-associated protein kinase 1, is a serine/threonine kinase that plays a crucial role in various cellular processes, including regulating the cytoskeleton, cell motility, and signal transduction. This enzyme is part of the Rho GTPase signaling pathway, which is vital for cell division, migration, and adhesion. Dysregulation of Rock1 has been implicated in several pathological conditions, such as cancer, cardiovascular diseases, and neurological disorders. Research into the recombinant expression of Rock1 has opened new avenues for understanding its functional mechanisms and interactions within the cell. By studying the structural and functional properties of Rock1 through recombinant technology, researchers can gain insights into its regulatory roles and potential as a therapeutic target. The production of Rock1 as a recombinant protein allows for detailed biochemical analyses and facilitates the development of specific inhibitors that could mitigate diseases associated with its overactivity. Therefore, Rock1 recombinant protein research not only aims to elucidate its biological functions but also seeks to harness this knowledge to create novel therapeutic strategies for diseases linked to Rho signaling pathway dysregulation.
