Analytical Data
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Gene name
DFNA5
- Application
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Alternative Names
2310037D07Rik; 4932441K13Rik; Deafness. autosomal dominant 5; Deafness. autosomal dominant 5 protein; DFNA5; DFNA5 gene; DFNA5_HUMAN; Dfna5h; EG14210; Fin15; ICERE 1; ICERE-1
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60443
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Expression Region
1-496aa
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AA Sequence
MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSPVVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCALGREHS
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Molecular Weight
81 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DFNA5, a gene associated with non-syndromic hearing loss, encodes a protein that plays a crucial role in the auditory system. Mutations or deletions in DFNA5 have been linked to autosomal dominant hearing impairment in various populations. Recent studies have highlighted the significance of DFNA5 in cochlear cell function and its involvement in apoptosis and cellular stress responses. The DFNA5 protein contains a unique structure that is essential for its function, and its disruption can lead to impaired auditory signaling pathways. Understanding the molecular mechanisms underlying DFNA5-related hearing loss is vital for developing targeted therapies and interventions. Research focusing on the recombinant expression of DFNA5 protein aims to elucidate its functional properties, interaction with other cellular proteins, and its role in hearing loss mechanisms. By generating recombinant DFNA5, scientists can explore potential therapies, such as gene therapy or small molecule drugs, that could restore hearing function or protect cochlear cells from degeneration. As a result, the study of DFNA5 recombinant protein continues to be a promising area in otology, with the potential to offer insights into treating genetic forms of hearing impairment.
