Analytical Data
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Gene name
DENND2D
- Application
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Alternative Names
2010308M01Rik; AI451421; DEN2D_HUMAN; DENN domain containing 2D; DENN domain-containing protein 2D; DENN/MADD domain containing 2D; DENND2D; FLJ22457; MGC6915; RGD1305120; RP5-1180E21.2
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H6A0
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Expression Region
1-471aa
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AA Sequence
MEGQVVGRVFRLFQRRLLQLRAGPPQDNSGEALKEPERAQEHSLPNFAGGQHFFEYLLVVSLKKKRSEDDYEPIITYQFPKRENLLRGQQEEEERLLKAIPLFCFPDGNEWASLTEYPRETFSFVLTNVDGSRKIGYCRRLLPAGPGPRLPKVYCIISCIGCFGLFSKILDEVEKRHQISMAVIYPFMQGLREAAFPAPGKTVTLKSFIPDSGTEFISLTRPLDSHLEHVDFSSLLHCLSFEQILQIFASAVLERKIIFLAEGLSTLSQCIHAAAALLYPFSWAHTYIPVVPESLLATVCCPTPFMVGVQMRFQQEVMDSPMEEVLLVNLCEGTFLMSVGDEKDILPPKLQDDILDSLGQGINELKTAEQINEHVSGPFVQFFVKIVGHYASYIKREANGQGHFQERSFCKALTSKTNRRFVKKFVKTQLFSLFIQEAEKSKNPPAGYFQQKILEYEEQKKQKKPREKTVK
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Molecular Weight
80.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DENND2D is a member of the DENN (differentially expressed in normal and neoplastic cells) domain-containing proteins, which play crucial roles in various cellular processes, including vesicular transport, cytoskeletal organization, and cell signaling. Emerging research indicates that DENND2D is particularly involved in the regulation of small GTPases, specifically in the Rab family, which are critical for intracellular trafficking and organelle dynamics. Its dysfunction has been implicated in several diseases, including cancer and neurodegenerative disorders, highlighting the importance of understanding its molecular mechanisms. Given the increasing interest in targeted therapies, DENND2D has garnered attention for its potential as a therapeutic target. Studies on recombinant DENND2D proteins aim to elucidate its structure-function relationship, explore its interaction with substrates, and dissect its role in cellular pathways. Furthermore, generating recombinant DENND2D provides valuable insights into its regulatory mechanisms and how alterations in its function might contribute to disease progression. This research not only enhances our understanding of DENND2D's biological role but also paves the way for novel therapeutic strategies aimed at modulating its activity in pathological contexts.
