Analytical Data
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Gene name
DENND1B
- Application
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Alternative Names
C1ORF18; C1orf218 ; Chromosome 1 open reading frame 218; Connecdenn 2; DEN1B_HUMAN; DENN domain containing protein 1B; DENN domain-containing protein 1B; DENN MADD domain containing 1B; DENND1B; DKFZp547O0715; FAM31B
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6P3S1
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Expression Region
1-396aa
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AA Sequence
MAAAPREEKRWPQPVFSNPVVLWKFPEDFGDQEILQSVPKFCFPFDVERVSQNQVGQHFTFVLTDIESKQRFGFCRLTSGGTICLCILSYLPWFEVYYKLLNTLADYLAKELENDLNETLRSLYNHPVPKANTPVNLSVHSYFIAPDVTGLPTIPESRNLTEYFVAVDVNNMLQLYASMLHERRIVIISSKLSTLTACIHGSAALLYPMYWQHIYIPVLPPHLLDYCCAPMPYLIGIHSSLIERVKNKSLEDVVMLNVDTNTLESPFSDLNNLPSDVVSALKNKLKKQSTATGDGVARAFLRAQAALFGYYRDALRYKPGEPITFCEESFVKHRSSVMKQFLETAINLQLFKQFIDGRLAKLNAGRGFSDVFEEEITSGGFCGGKDKLQYDYPFSQ
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Molecular Weight
71.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DENND1B, a member of the DENN (differentially expressed in normal and neoplastic cells) protein family, is known for its role as a guanine nucleotide exchange factor (GEF) that activates Rab GTPases, key regulators of vesicular trafficking and cell signaling. Research has shown that mutations in the DENND1B gene are associated with various developmental disorders and immune system abnormalities, particularly in influencing microRNA regulation and endocytic processes. The study of DENND1B recombinant proteins has gained attention due to their potential therapeutic implications in modulating cellular functions linked to these diseases. Evaluating the biochemical properties and functional roles of DENND1B proteins can provide insights into their mechanisms of action and impact on cellular pathways, thus offering a pathway for developing targeted treatments for related disorders. This research not only enhances our understanding of basic cellular processes, but also opens avenues for innovative strategies in drug design and treatment interventions, particularly in conditions where vesicular trafficking is disrupted. As the implications of DENND1B dysfunction in both health and disease become more evident, the exploration of its recombinant forms offers promising opportunities for uncovering novel therapeutic targets and advancing our knowledge of complex cellular dynamics.
