Analytical Data
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Gene name
SMYD1
- Application
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Alternative Names
BOP; CD8 beta opposite; CD8b opposite; Histone lysine N methyltransferase SMYD1; KMT3D; SET and MYND domain containing 1; SET and MYND domain-containing protein 1; SMYD1; SMYD1_HUMAN; Zinc finger MYND domain containing 18; ZMYND18; ZMYND22
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8NB12
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Expression Region
1-490 aa
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AA Sequence
MTIGRMENVE VFTAEGKGRG LKATKEFW DIIFAERAYS AVVFDSLVNF VCHTCFKRQE KLHRCGQCKF AHYCDRTCQK DAWLNHKNEC SAIKRYGKVP NENIRLRI MWRVEREGTG LTEGCLVSVD DLQNHVEHFG EEEQKDLRVD VDTFLQYWPP QSQQFSMQYI SHIFGVINCN GFTLSDQRGL QAVGVGIFPN LGLVNHDCWP NCTVIFNNGN HEAVKSMFHT QMRIELRALG KISEGEELTV SYIDFLNVSE ERKRQLKKQY YFDCTCEHCQ KKLKDDLFLG VKDNPKPSQE VVKEMIQFSK DTLEKIDKAR SEGLYHEVVK LCRECLEKQE PVFADTNIYM LRMLSIVSEV LSYLQAFEEA SFYARRMVDG YMKLYHPNNA QLGMAVMRAG LTNWHAGNIE VGHGMICKAY AILLVTHGPS HPITKDLEAM RVQTEMELRM FRQNEFMYYK MRELNNQP MQVMAEPSNE PSPALFHKKQ
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Molecular Weight
56.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SMYD1, a member of the SET and MYND domain-containing (SMYD) family of proteins, has garnered significant interest in the field of epigenetics and cardiac biology. It acts as a lysine methyltransferase, specifically targeting histone and non-histone substrates, thereby regulating gene expression and cellular functions. Research has indicated that SMYD1 plays a crucial role in cardiac development and function, with its expression being particularly enriched in heart tissues. Dysregulation of SMYD1 has been implicated in various cardiovascular diseases, making it a potential therapeutic target. The study of SMYD1 recombinant proteins facilitates the understanding of its enzymatic activity, substrate specificity, and biological roles. By employing techniques such as site-directed mutagenesis and structural biology, researchers aim to elucidate the mechanisms by which SMYD1 influences gene regulation and cellular processes in the heart. This burgeoning area of research also holds promise for developing innovative strategies to treat heart diseases linked to SMYD1 dysfunction, highlighting its importance in both basic and applied biomedical sciences.
