Analytical Data
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Gene name
DCAF12L1
- Application
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Alternative Names
DCAF12L1; WDR40B; DDB1- and CUL4-associated factor 12-like protein 1; WD repeat-containing protein 40B
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5VU92
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Expression Region
1-463aa
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AA Sequence
MAQQQTGSRKRKAPAVEAGAESSPSQGLAAADGEGPLLLKRQRRPATYRSMAHYLKVREVGGWGPARLQGFDGELRGYAVQRLPELLTERQLELGTVNKVFASQWLNSRQVVCGTKCNTLFVVDVESGHIARIPLLRDSEARLAQDQQGCGIHAIELNPSKTLLATGGENPNSLAIYQLPSLDPLCLGDRHGHKDWIFAVAWLSDTVAVSGSRDGTVALWRMDPDKFDDTVAWHSEVGLPVYAHIRPRDVEAIPRAIINPSNRKVRALACGGKNQELGAVSLDGYFHLWKAGSALSRLLSIRLPYFRDNVCLTYCDDMSVYAVGSHSHVSFLDLRQDQQNIRPLCSREGGTGVRSLSFYRHIITVGTGQGSLLFYDVRAQKFLEERASATLESSSGPARRKLRLACGRGWLNHNDFWVNYFGGMEVFPNALYTHCYNWPEMKLFVAGGPLPAGLHGNYAGLWS
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Molecular Weight
51 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DCAF12L1, a member of the DDB1-CUL4-ROC1 E3 ubiquitin ligase complex, plays a crucial role in various cellular processes, including protein degradation and cellular signaling. Research into DCAF12L1 has gained attention due to its involvement in regulating key cellular functions such as cell cycle progression, apoptosis, and response to DNA damage. Abnormal expression or mutations in DCAF12L1 have been associated with various diseases, including cancer, making it a potential biomarker and therapeutic target. The ability to produce recombinant DCAF12L1 protein allows for in-depth studies of its biochemical properties, interactions with other proteins, and functional implications in cellular pathways. By understanding the molecular mechanisms governed by DCAF12L1, researchers aim to elucidate its role in disease progression and therapeutic resistance, ultimately contributing to the development of innovative treatment strategies. As the field of protein engineering and synthetic biology advances, the study of recombinant DCAF12L1 continues to unfold, revealing insights that may lead to significant breakthroughs in biomedical research and clinical applications.
