Analytical Data
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基因名
SMG8
- Application
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别名
SMG8; ABC2; C17orf71; Protein SMG8; Amplified in breast cancer gene 2 protein; Protein smg-8 homolog
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8ND04
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表达区间
1-642 aa
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氨基酸序列
MLLDQLRSHCTVKDPESLLVPAPLSGPRRYQVMRQHSRQQLSFHIDSSSSSSSGQLVDFTLREFLWQHVELVLSKKGFDDSVGRNPQPSHFELPTYQKWISAASKLYEVAIDGKEEDLGSPTGELTSKILSSIKVLEGFLDIDTKFSENRCQKALPMAHSAYQSNLPHNYTMTVHKNQLAQALRVYSQHARGPAFHKYAMQLHEDCYKFWSNGHQLCEERSLTDQHCVHKFHSLPKSGEKPEADRNPPVLYHNSRARSTGACNCGRKQAPRDDPFDIKAANYDFYQLLEEKCCGKLDHINFPVFEPSTPDPAPAKNESSPAPPDSDADKLKEKEPQTQGESTSLSLALSLGQSTDSLGTYPADPQAGGDNPEVHGQVEVKTEKRPNFVDRQASTVEYLPGMLHSNCPKGLLPKFSSWSLVKLGPAKSYNFHTGLDQQGFIPGTNYLMPWDIVIRTRAEDEGDLDTNSWPAPNKAIPGKRSAVVMGRGRRRDDIARAFVGFEYEDSRGRRFMCSGPDKVMKVMGSGPKESALKALNSDMPLYILSSSQGRGLKPHYAQLMRLFVVVPDAPLQIILMPQVQPGPPPCPVFYPEKQEITLPPDGLWVLRFPYAYVTERGPCFPPKENVQLMSYKVLRGVLKAVTQ
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分子量
70.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SMG8 (Suppressor with Morphogenetic effect on genitalia 8) is a vital component of the nonsense-mediated mRNA decay (NMD) pathway, which is crucial for maintaining mRNA surveillance and quality control in eukaryotic cells. This protein, part of a larger complex that includes SMG1, SMG5, SMG6, and SMG7, plays a significant role in recognizing and degrading mRNAs that contain premature stop codons, thereby preventing the production of truncated and potentially harmful proteins. Research into SMG8 and its reconstituted protein interactions has gained momentum due to its implications in various biological processes and disease states, including cancer and genetic disorders associated with dysregulated mRNA metabolism. The study of SMG8's structure and function, particularly through the development of recombinant forms of the protein, is essential for elucidating its role in NMD and understanding its interactions with other proteins in the decay machinery. Advances in techniques such as X-ray crystallography and cryo-electron microscopy have facilitated detailed investigations into SMG8's molecular dynamics and its contributions to cellular responses to stress and developmental cues. Thus, examining the reconstituted SMG8 protein not only enhances our comprehension of mRNA surveillance mechanisms but may also provide insights into therapeutic strategies for conditions arising from NMD pathway dysfunction.
