Analytical Data
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基因名
DCAF4
- Application
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别名
DCAF4；WDR21；WDR21A；DDB1- and CUL4-associated factor 4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8WV16
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表达区间
1-495aa
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氨基酸序列
MNKSRWQSRRRHGRRSHQQNPWFRLRDSEDRSDSRAAQPAHDSGHGDDESPSTSSGTAGTSSVPELPGFYFDPEKKRYFRLLPGHNNCNPLTKESIRQKEMESKRLRLLQEEDRRKKIARMGFNASSMLRKSQLGFLNVTNYCHLAHELRLSCMERKKVQIRSMDPSALASDRFNLILADTNSDRLFTVNDVKVGGSKYGIINLQSLKTPTLKVFMHENLYFTNRKVNSVCWASLNHLDSHILLCLMGLAETPGCATLLPASLFVNSHPGIDRPGMLCSFRIPGAWSCAWSLNIQANNCFSTGLSRRVLLTNVVTGHRQSFGTNSDVLAQQFALMAPLLFNGCRSGEIFAIDLRCGNQGKGWKATRLFHDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCVRQYEGHVNEYAYLPLHVHEEEGILVAVGQDCYTRIWSLHDARLLRTIPSPYPASKADIPSVAFSSRLGGSRGAPGLLMAVGQDLYCYSYS
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分子量
71.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DCAF4 (DDB1 and CUL4 associated factor 4) is a protein that plays a critical role in the cellular response to various stresses, including DNA damage and protein misfolding. It is part of the DDB1-CUL4-RING ubiquitin ligase complex, which is essential for targeting substrates for ubiquitination, a process that regulates protein stability and function. Research has shown that DCAF4 is involved in the modulation of several key cellular pathways, such as cell cycle progression and apoptosis, highlighting its importance in maintaining cellular homeostasis. The dysfunction of DCAF4 has been associated with various diseases, including cancer, where altered ubiquitination processes can contribute to tumorigenesis. Furthermore, understanding the structural and functional characteristics of DCAF4 and its interactions with other cellular components is crucial for uncovering its mechanisms in cellular regulation. As a result, studies involving the recombinant expression of DCAF4 have gained momentum, aiming to elucidate its role in protein degradation pathways and explore its potential as a therapeutic target in diseases characterized by aberrant ubiquitination. Insights gained from these studies could pave the way for novel strategies in disease treatment, ultimately contributing to the fields of molecular biology and therapeutic development.
