Analytical Data
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Gene name
CYP4F12
- Application
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Alternative Names
CYP4F12; UNQ568/PRO1129; Cytochrome P450 4F12; CYPIVF12
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HCS2
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Expression Region
1-524aa
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AA Sequence
MSLLSLPWLGLRPVAMSPWLLLLLVVGSWLLARILAWTYAFYNNCRRLQCFPQPPKRNWFWGHLGLITPTEEGLKDSTQMSATYSQGFTVWLGPIIPLIVLCHPDTIRSITNASAAIAPKDNLFIRFLKPWLGEGILLSGGDKWSRHRRMLTPAFHFNILKSYITIFNKSANIMLDKWQHLASEGSSCLDMFEHISLMTLDSLQKCIFSFDSHCQERPSEYIATILELSALVEKRSQHILQHMDFLYYLSHDGRRFHRACRLVHDFTDAVIRERRRTLPTQGIDDFFKDKAKSKTLDFIDVLLLSKDEDGKALSDEDIRAEADTFMFGGHDTTASGLSWVLYNLARHPEYQERCRQEVQELLKDRDPKEIEWDDLAQLPFLTMCVKESLRLHPPAPFISRCCTQDIVLPDGRVIPKGITCLIDIIGVHHNPTVWPDPEVYDPFRFDPENSKGRSPLAFIPFSAGPRNCIGQAFAMAEMKVVLALMLLHFRFLPDHTEPRRKLELIMRAEGGLWLRVEPLNVSLQ
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Molecular Weight
83.38 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CYP4F12 is a member of the cytochrome P450 superfamily of enzymes, which are crucial in the metabolism of fatty acids and various endogenous and exogenous compounds. Research into CYP4F12 has gained momentum due to its potential roles in pharmacology and toxicology, particularly regarding drug metabolism and the biotransformation of xenobiotics. Given its involvement in the metabolism of arachidonic acid and other fatty acids, CYP4F12 may play a significant role in regulating inflammatory processes and homeostasis, making it a target of interest for understanding various disease mechanisms, including cardiovascular diseases and inflammatory disorders. Additionally, its enzymatic activities can influence the efficacy and safety of therapeutic agents. As such, studies focusing on the recombinant expression of CYP4F12 not only allow for detailed investigations into its catalytic properties but also facilitate the development of targeted drugs and the assessment of drug interactions. Furthermore, the exploration of CYP4F12 polymorphisms can provide insights into individual variability in drug response, highlighting its importance in personalized medicine. Thus, the research surrounding recombinant CYP4F12 sheds light on its biological significance and potential applications in pharmacotherapy and disease management.
