Analytical Data
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Gene name
CYP2J2
- Application
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Alternative Names
Albendazole monooxygenase hydroxylating;Albendazole monooxygenase sulfoxide-forming;Arachidonic acid epoxygenase;CYPIIJ2;Hydroperoxy icosatetraenoate isomerase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P51589
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Expression Region
33-502aa
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AA Sequence
DFLKRRRPKNYPPGPWRLPFLGNFFLVDFEQSHLEVQLFVKKYGNLFSLELGDISAVLITGLPLIKEALIHMDQNFGNRPVTPMREHIFKKNGLIMSSGQAWKEQRRFTLTALRNFGLGKKSLEERIQEEAQHLTEAIKEENGQPFDPHFKINNAVSNIICSITFGERFEYQDSWFQQLLKLLDEVTYLEASKTCQLYNVFPWIMKFLPGPHQTLFSNWKKLKLFVSHMIDKHRKDWNPAETRDFIDAYLKEMSKHTGNPTSSFHEENLICSTLDLFFAGTETTSTTLRWALLYMALYPEIQEKVQAEIDRVIGQGQQPSTAARESMPYTNAVIHEVQRMGNIIPLNVPREVTVDTTLAGYHLPKGTMILTNLTALHRDPTEWATPDTFNPDHFLENGQFKKREAFMPFSIGKRACLGEQLARTELFIFFTSLMQKFTFRPPNNEKLSLKFRMGITISPVSHRLCAVPQV
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Molecular Weight
61.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CYP2J2 is a member of the cytochrome P450 superfamily of enzymes, primarily found in the human liver and heart, known for its role in the metabolism of various endogenous and exogenous compounds. This enzyme catalyzes the oxidation of long-chain fatty acids and plays a significant role in the bioactivation of certain drugs, as well as the metabolism of the therapeutic agent Sunitinib. The functional significance of CYP2J2 extends beyond drug metabolism, as it is involved in the production of epoxyeicosatrienoic acids (EETs), which possess vasodilatory, anti-inflammatory, and cardioprotective effects. Dysfunction or altered expression of CYP2J2 has been linked to various cardiovascular diseases, making it a potential biomarker for cardiovascular health and a target for therapeutic interventions. Recent studies have focused on the recombinant expression of CYP2J2 to facilitate the characterization of its enzymatic activity and substrate specificity. These efforts aim to understand the impact of genetic polymorphisms and regulatory mechanisms that modify CYP2J2 function, ultimately contributing to advancements in precision medicine and personalized therapy strategies. The recombinant protein studies not only enhance our understanding of CYP2J2's pharmacogenomic relevance but also pave the way for innovative drug development approaches targeting CYP2J2's metabolic pathways.
