Analytical Data
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Gene name
CYP2F1
- Application
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Alternative Names
CYP2F1Cytochrome P450 2F1; EC 1.14.14.1; CYPIIF1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P24903
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Expression Region
1-491aa
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AA Sequence
MDSISTAILL LLLALVCLLL TLSSRDKGKL PPGPRPLSIL GNLLLLCSQD MLTSLTKLSK EYGSMYTVHL GPRRVVVLSG YQAVKEALVD QGEEFSGRGD YPAFFNFTKG NGIAFSSGDR WKVLRQFSIQ ILRNFGMGKR SIEERILEEG SFLLAELRKT EGEPFDPTFV LSRSVSNIIC SVLFGSRFDY DDERLLTIIR LINDNFQIMS SPWGELYDIF PSLLDWVPGP HQRIFQNFKC LRDLIAHSVH DHQASLDPRS PRDFIQCFLT KMAEEKEDPL SHFHMDTLLM TTHNLLFGGT KTVSTTLHHA FLALMKYPKV QARVQEEIDL VVGRARLPAL KDRAAMPYTD AVIHEVQRFA DIIPMNLPHR VTRDTAFRGF LIPKGTDVIT LLNTVHYDPS QFLTPQEFNP EHFLDANQSF KKSPAFMPFS AGRRLCLGES LARMELFLYL TAILQSFSLQ PLGAPEDIDL TPLSSGLGNL PRPFQLCLRP R
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Molecular Weight
55.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CYP2F1, a member of the cytochrome P450 family, is an important enzyme involved in the metabolism of various endogenous and exogenous compounds, including certain drugs and environmental toxins. This enzyme is primarily expressed in the respiratory system, particularly in the lung, indicating its potential role in pulmonary metabolism and detoxification processes. The study of CYP2F1 is significant due to its implications in pharmacogenetics, as variations in its expression or activity may influence individual responses to therapeutic drugs and susceptibility to lung diseases, including asthma and cancer. Additionally, CYP2F1 has been implicated in the bioactivation of procarcinogens found in tobacco smoke, linking its activity to smoking-related pathologies. Research into the recombinant expression and functional characterization of CYP2F1 can provide valuable insights into its biochemical properties and substrate specificity, facilitating a better understanding of its role in drug metabolism and environmental interactions. Furthermore, such studies can aid in the development of targeted therapies and personalized medicine approaches, enhancing treatment efficacy and safety for individuals with varying CYP2F1 profiles. Overall, the investigation of CYP2F1 and its recombinant protein forms holds promise for advancing our comprehension of metabolic processes and their clinical relevance in human health.
