Analytical Data
-
基因名
DNMT1
- Application
-
别名
DNMT1；AIM；CXXC9；DNMT；DNA (cytosine-5)-methyltransferase 1
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P26358-2
-
表达区间
2-1632aa
-
氨基酸序列
PARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHE FLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNR EVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKRSR DPPASASQVTGIRAEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESE RAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEE ERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKK HRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEK KMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEK LSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFF SGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAE YILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTV PPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLI KLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQ IEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQA CQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVG EAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWED SSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVI YKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPT EDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRV GDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNL DAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASY HADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYF LEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIK LPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGST VFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRF NSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLR CLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFA PRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALE ISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSD WRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKAC DPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGK QGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLA KAIGLEIKLCMLAKARESASAKIKEEEAAKD
-
分子量
211 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DNMT1 (DNA methyltransferase 1) is a crucial enzyme involved in the maintenance of DNA methylation patterns, which play a significant role in the regulation of gene expression and cellular processes such as differentiation, development, and genomic stability. Abnormal DNMT1 activity is linked to various diseases, including cancer, where altered methylation can lead to gene silencing or activation that contributes to tumor progression. Research into DNMT1 recombinant proteins has gained momentum as they offer valuable tools for understanding the enzyme's mechanism of action, substrate specificity, and interaction with other cellular components. By producing and studying these recombinant proteins, researchers aim to elucidate the role of DNMT1 in epigenetic regulation and its implications in diseases. Moreover, the ability to manipulate DNMT1 activity using recombinant approaches can pave the way for novel therapeutic strategies targeting methylation changes in cancer and other disorders, enhancing the potential for precision medicine. This growing interest underscores the importance of DNMT1 as a focal point for both fundamental research and clinical applications, emphasizing the need for detailed investigations into its functional characteristics and regulatory networks.
