Analytical Data
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Gene name
EP300
- Application
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Alternative Names
EP300；P300；Histone acetyltransferase p300
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q09472
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Expression Region
985-1810aa
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AA Sequence
QAIAEKQPSQEVKMEAKMEVDQPEPADTQPEDISESKVEDCKMESTETEE RSTELKTEIKEEEDQPSTSATQSSPAPGQSKKKIFKPEELRQALMPTLEA LYRQDPESLPFRQPVDPQLLGIPDYFDIVKSPMDLSTIKRKLDTGQYQEP WQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQEIDPVMQSLGYCC GRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQGESVS LGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEI IWPAGFVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQN HPESGEVTVRVVHASDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFE EIDGVDLCFFGMHVQEYGSDCPPPNQRRVYISYLDSVHFFRPKCLRTAVY HEILIGYLEYVKKLGYTTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQ EWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLE ESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVD PDPLIPCDLMDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD RFVYTCNECKHHVETRWHCTVCEDYDLCITCYNTKNHDHKMEKLGLGLDD ESNNQQAAATQSPGDSRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRV VQHTKGCKRKTNGGCPICKQLIALCCYHAKHCQENKCPVPFCLNIK
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Molecular Weight
125 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EP300, also known as E1A-binding protein p300, is a transcriptional co-activator that plays a crucial role in regulating gene expression involved in various cellular processes, including cell growth, differentiation, and apoptosis. The study of EP300 has gained significant attention due to its implications in cancer biology and other diseases. Mutations and dysregulation of EP300 have been associated with several types of cancer, such as leukemia and solid tumors, highlighting its potential as a therapeutic target. Additionally, EP300 interacts with numerous transcription factors and chromatin-modifying complexes, making it essential for the modulation of gene expression in response to external stimuli. The recombinant expression of EP300 protein allows researchers to study its structure-function relationships, post-translational modifications, and interactions with other cellular molecules in a controlled environment. By producing EP300 as a recombinant protein, scientists can investigate its role in epigenetic regulation and its impact on the transcriptional landscape. This research is vital for understanding the molecular mechanisms underlying cellular processes and could lead to novel strategies for cancer therapy and other diseases where EP300 is implicated. The development of small molecules or peptides that can modulate EP300 activity represents a promising area of therapeutic exploration, making the study of this protein not only relevant to basic research but also to the development of future clinical applications. Through ongoing research, understanding the biology of EP300 is expected to provide insights into its potential as a biomarker and therapeutic target, enriching our knowledge of cancer biology and beyond.
