Analytical Data
-
基因名
EP300
- Application
-
别名
EP300；P300；Histone acetyltransferase p300
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q09472
-
表达区间
985-1810aa
-
氨基酸序列
QAIAEKQPSQEVKMEAKMEVDQPEPADTQPEDISESKVEDCKMESTETEE RSTELKTEIKEEEDQPSTSATQSSPAPGQSKKKIFKPEELRQALMPTLEA LYRQDPESLPFRQPVDPQLLGIPDYFDIVKSPMDLSTIKRKLDTGQYQEP WQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQEIDPVMQSLGYCC GRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQGESVS LGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEI IWPAGFVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQN HPESGEVTVRVVHASDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFE EIDGVDLCFFGMHVQEYGSDCPPPNQRRVYISYLDSVHFFRPKCLRTAVY HEILIGYLEYVKKLGYTTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQ EWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLE ESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVD PDPLIPCDLMDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD RFVYTCNECKHHVETRWHCTVCEDYDLCITCYNTKNHDHKMEKLGLGLDD ESNNQQAAATQSPGDSRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRV VQHTKGCKRKTNGGCPICKQLIALCCYHAKHCQENKCPVPFCLNIK
-
分子量
125 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EP300, also known as E1A-binding protein p300, is a transcriptional co-activator that plays a crucial role in regulating gene expression involved in various cellular processes, including cell growth, differentiation, and apoptosis. The study of EP300 has gained significant attention due to its implications in cancer biology and other diseases. Mutations and dysregulation of EP300 have been associated with several types of cancer, such as leukemia and solid tumors, highlighting its potential as a therapeutic target. Additionally, EP300 interacts with numerous transcription factors and chromatin-modifying complexes, making it essential for the modulation of gene expression in response to external stimuli. The recombinant expression of EP300 protein allows researchers to study its structure-function relationships, post-translational modifications, and interactions with other cellular molecules in a controlled environment. By producing EP300 as a recombinant protein, scientists can investigate its role in epigenetic regulation and its impact on the transcriptional landscape. This research is vital for understanding the molecular mechanisms underlying cellular processes and could lead to novel strategies for cancer therapy and other diseases where EP300 is implicated. The development of small molecules or peptides that can modulate EP300 activity represents a promising area of therapeutic exploration, making the study of this protein not only relevant to basic research but also to the development of future clinical applications. Through ongoing research, understanding the biology of EP300 is expected to provide insights into its potential as a biomarker and therapeutic target, enriching our knowledge of cancer biology and beyond.
