Analytical Data
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Gene name
DYNC2LI1
- Application
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Alternative Names
DYNC2LI1；D2LIC；LIC3；Cytoplasmic dynein 2 light intermediate chain 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8TCX1
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Expression Region
1-352aa
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AA Sequence
MPSETLWEIAKAEVEKRGINGSEGDGAEIAEKFVFFIGSKNGGKTTIILRCLDRDEPPKPTLALEYTYGRRAKGHNTPKDIAHFWELGGGTSLLDLISIPITGDTLRTFSLVLVLDLSKPNDLWPTMENLLQATKSHVDKVIMKLGKTNAKAVSEMRQKIWNNMPKDHPQDHELIDPFPVPLVIIGSKYDVFQDFESEKRKVICKTLRFVAHYYGASLMFTSKSEALLLKIRGVINQLAFGIDKSKSICVDQNKPLFITAGLDSFGQIGSPPVPENDIGKLHAHSPMELWKKVYEKLFPPKSINTLKDIKDPARDPQYAENEVDEMRIQKDLELEQYKRSSSKSWKQIELDS
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Molecular Weight
44.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DYNC2LI1 is a critical component of the dynein motor protein complex, which plays a vital role in intracellular transport, particularly in the movement of organelles, vesicles, and other cargo along microtubules. Dysfunctional dynein activity has been implicated in various neurodegenerative diseases, such as Alzheimer’s and Huntington’s disease, as well as in congenital disorders affecting neuronal development. The study of DYNC2LI1, a light intermediate chain of cytoplasmic dynein-2, has gained attention due to its essential role in regulating dynein motor activity and cargo binding specificity. Researchers focus on reconstituting DYNC2LI1 protein to elucidate its structural properties and functional mechanisms, aiming to understand how mutations in this protein can lead to dysfunction in lysosomal trafficking and ciliary transport. Investigating DYNC2LI1 through recombinant protein techniques provides insights into its interactions with other protein partners within the dynein complex, thus contributing to a deeper understanding of the molecular pathways involved in cellular transport processes. Moreover, such studies could pave the way for therapeutic strategies targeting dynein-related pathologies. The growing interest in DYNC2LI1 reflects a broader commitment to unraveling the complexities of motor protein function in health and disease.
