Analytical Data
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Gene name
PIK3R5
- Application
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Alternative Names
PIK3R5；Phosphatidylinositol 4.5-bisphosphate 3-kinase catalytic subunit gamma isoform
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8WYR1
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Expression Region
1-494aa
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AA Sequence
MDSGYVEDSEESSSEWPWRRGSQERRGHRRPGQKFIRIYKLFKSTSQLVLRRDSRSLEGSSDTALPLRRAGSLCSPLDEPVSPPSRAQRSRSLPQPKLGTQLPSWLLAPASRPQRRRPFLSGDEDPKASTLRVVVFGSDRISGKVARAYSNLRRLENNRPLLTRFFKLQFFYVPVKRSHGTSPGACPPPRSQTPSPPTDSPRHASPGELGTTPWEESTNDISHYLGMLDPWYERNVLGLMHLPPEVLCQQSLKAEAQALEGSPTQLPILADMLLYYCRFAARPVLLQVYQTELTFITGEKTTEIFIHSLELGHSAATRAIKASGPGSKRLGIDGDREAVPLTLQIIYSKGAISGRSRWSNLEKVCTSVNLNKACRKQEELDSSMEALTLNLTEVVKRQNSKSKKGFNQISTSQIKVDKVQIIGSNSCPFAVCLDQDERKILQSVVRCEVSPCYKPEKSDLSSPPQTPPDLPAQAAPDLCSLLCLPIMTFSGALP
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Molecular Weight
70.9kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PIK3R5, also known as p85 beta, is a regulatory subunit of phosphoinositide 3-kinases (PI3Ks), which play critical roles in various cellular processes, including growth, metabolism, and survival. The PI3K signaling pathway is implicated in numerous physiological functions and is often dysregulated in cancers and other diseases. Unlike its more extensively studied counterpart, PIK3R1 (p85 alpha), PIK3R5 has received comparatively less attention, making it an intriguing subject for research. Preliminary studies suggest that PIK3R5 may have distinct regulatory functions and potential implications in tumorigenesis, inflammation, and metabolic disorders. Understanding the structure, function, and interactions of PIK3R5 can provide valuable insights into its contribution to these pathological conditions. Moreover, the generation of recombinant PIK3R5 protein allows for detailed biochemical and biophysical analyses, facilitating the exploration of its roles in the PI3K pathway. This research can also aid in the development of targeted therapies that manipulate PIK3R5 activity, offering new avenues for treating diseases associated with PI3K dysregulation. Given the growing interest in PI3K-related pathways in cancer and other diseases, further investigation into PIK3R5 is not only timely but also essential for advancing our understanding of cellular signaling mechanisms and therapeutic interventions.
