Analytical Data
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基因名
ACF
- Application
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别名
ACF；ACF；ASP；APOBEC1 complementation factor
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NRL2
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表达区间
1415-1545aa
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氨基酸序列
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSEPSPVTLGRRSSGRQGGVH ELSAFEQLVVELVRHDDSWPFLKLVSKIQVPDYYDIIKKPIALNIIREKV NKCEYKLASEFIDDIELMFSNCFEYNPRNTSEAKAGTRLQAFFHIQAQKL GLHVTPSNVDQV
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分子量
42 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACF (ATP-dependent chromatin assembly factor) is a crucial multiprotein complex involved in the dynamic organization of chromatin, which plays a fundamental role in regulating gene expression and maintaining genomic stability. The study of ACF and its recombinant proteins has gained significant attention in recent years due to its importance in various cellular processes, including DNA replication, repair, and transcription regulation. ACF is composed of several subunits that work together to facilitate the ATP-dependent assembly of nucleosomes, thus influencing chromatin structure and function. Research into the structural and functional properties of ACF recombinant proteins has provided insights into how chromatin remodeling is coordinated at the molecular level, helping to elucidate the mechanisms underlying epigenetic regulation and chromatin dynamics. Additionally, understanding the role of ACF in disease contexts, such as cancer and developmental disorders, underscores the relevance of this research in potential therapeutic applications. By harnessing recombinant protein techniques, scientists aim to dissect the intricate interplay between ACF and other chromatin-associated factors, advancing our knowledge of cellular mechanisms and paving the way for novel strategies to manipulate gene expression for therapeutic purposes.
