Analytical Data
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Gene name
ABHD5
- Application
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Alternative Names
ABHD5；NCIE2；1-acylglycerol-3-phosphate O-acyltransferase ABHD5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8WTS1
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Expression Region
2-349aa
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AA Sequence
AAEEEEVDSADTGERSGWLTGWLPTWCPTSISHLKEAEEKMLKCVPCTYKKEPVRISNGNKIWTLKFSHNISNKTPLVLLHGFGGGLGLWALNFGDLCTNRPVYAFDLLGFGRSSRPRFDSDAEEVENQFVESIEEWRCALGLDKMILLGHNLGGFLAAAYSLKYPSRVNHLILVEPWGFPERPDLADQDRPIPVWIRALGAALTPFNPLAGLRIAGPFGLSLVQRLRPDFKRKYSSMFEDDTVTEYIYHCNVQTPSGETAFKNMTIPYGWAKRPMLQRIGKMHPDIPVSVIFGARSCIDGNSGTSIQSLRPHSYVKTIAILGAGHYVYADQPEEFNQKVKEICDTVD
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Molecular Weight
55.0 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ABHD5 (Alpha/Beta Hydrolase Domain Containing 5) is a critical protein involved in lipid metabolism, particularly in the regulation of intracellular lipid droplets and triacylglycerol hydrolysis. Its function is closely associated with the activation of adipose triglyceride lipase (ATGL), an enzyme that catalyzes the breakdown of stored fats. The study of ABHD5 has gained significant attention due to its role in various physiological processes and its implications in metabolic disorders. For instance, mutations in the ABHD5 gene have been linked to congenital lipodystrophy, a condition characterized by the abnormal distribution of body fat and severe metabolic dysregulation. Furthermore, ABHD5 is implicated in diverse cellular processes, including inflammation, energy homeostasis, and the response to oxidative stress. Understanding the structure and function of recombinant ABHD5 protein can provide insights into its mechanism of action and its interactions with other metabolic enzymes. This research is pivotal for developing therapeutic strategies to combat obesity, diabetes, and related metabolic diseases, highlighting the importance of ABHD5 as a potential target for drug development. Overall, the exploration of ABHD5's role in lipid metabolism supports a broader understanding of energy balance in human health, making it a focal point for future biochemical and pharmacological studies.
