Analytical Data
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Gene name
SLC22A8
- Application
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Alternative Names
hOAT3; OAT3 ; Organic anion transporter 3; S22A8_HUMAN; SLC22A8; Solute carrier family 22 (organic anion transporter); member 8; Solute carrier family 22 member 8
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8TCC7
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Expression Region
1-542 aa
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AA Sequence
MTFSEILDRVGSMGHFQFLHVAILGLPILNMANHNLLQIFTAATPVHHCRPPHNASTGPWVLPMGPNGKPERCLRFVHPPNASLPNDTQRAMEPCLDGWVYNSTKDSIVTEWDLVCNSNKLKEMAQSIFMAGILIGGLVLGDLSDRFGRRPILTCSYLLLAASGSGAAFSPTFPIYMVFRFLCGFGISGITLSTVILNVEWVPTRMRAIMSTALGYCYTFGQFILPGLAYAIPQWRWLQLTVSIPFFVFFLSSWWTPESIRWLVLSGKSSKALKILRRVAVFNGKKEEGERLSLEELKLNLQKEISLAKAKYTASDLFRIPMLRRMTFCLSLAWFATGFAYYSLAMGVEEFGVNLYILQIIFGGVDVPAKFITILSLSYLGRHTTQAAALLLAGGAILALTFVPLDLQTVRTVLAVFGKGCLSSSFSCLFLYTSELYPTVIRQTGMGVSNLWTRVGSMVSPLVKITGEVQPFIPNIIYGITALLGGSAALFLPETLNQPLPETIEDLENWSLRAKKPKQEPEVEKASQRIPLQPHGPGLGSS
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Molecular Weight
86.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SLC22A8, also known as OCTN1 (Organic Cation/Carnitine Transporter 1), is a member of the SLC22 transporter family and plays a crucial role in the transport of various substrates, including organic cations and carnitine. This transporter is particularly important for nutrient uptake and metabolic regulation in human cells. Research into SLC22A8 has gained momentum due to its implications in various physiological processes and potential links to several diseases, such as obesity, diabetes, and kidney disorders. The identification of SLC22A8 as a principal facilitator of carnitine transport has underscored its relevance in fatty acid metabolism and energy homeostasis, making it a target for therapeutic interventions. Moreover, variations in the SLC22A8 gene have been associated with altered transporter activity, influencing drug pharmacokinetics and responsiveness. Consequently, the recombinant expression of SLC22A8 has been pursued in order to study its functional properties, substrate specificity, and regulatory mechanisms in detail. By utilizing recombinant protein techniques, researchers aim to elucidate the structure-function relationships of this transporter, which can provide insights into its role in health and disease, as well as inform the design of novel pharmacological agents targeting SLC22A8-associated pathways.
