Analytical Data
-
Gene name
CPSF5
- Application
-
Alternative Names
CFIM 25; CFIM25; Cleavage and polyadenylation specific factor 5 25 kD subunit; Cleavage and polyadenylation specific factor 5 25 kDa; Cleavage and polyadenylation specific factor 5; Cleavage and polyadenylation specificity factor 25 kDa subunit; Cleavage and polyadenylation specificity factor subunit 5; Cleavage factor Im complex 25 kDa subunit
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O43809
-
Expression Region
1-227aa
-
AA Sequence
MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRFNFIYN
-
Molecular Weight
50.71 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CPSF5, also known as cleavage and polyadenylation specificity factor subunit 5, plays a crucial role in the post-transcriptional regulation of gene expression by participating in the polyadenylation of mRNA precursors. This protein is a component of the CPSF complex, which is essential for the cleavage of pre-mRNA and the addition of a poly(A) tail, a modification that stabilizes mRNA and facilitates its export from the nucleus to the cytoplasm. Alterations in CPSF5 function can lead to various cellular dysfunctions and have been implicated in multiple diseases, including cancers and genetic disorders. The study of CPSF5 as a recombinant protein enables researchers to dissect its specific interactions and regulatory mechanisms in greater detail, facilitating the understanding of its role in mRNA processing and stability. Additionally, the ability to produce and purify CPSF5 in vitro allows for the development of potential therapeutic strategies aimed at modulating gene expression through the manipulation of mRNA polyadenylation dynamics. Thus, ongoing research into CPSF5 is critical for elucidating the complexities of gene regulation and may offer insights into novel approaches for treating diseases associated with dysregulated gene expression.
