Analytical Data
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基因名
CHEK1
- Application
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别名
CHEK1；CHK1；Serine/threonine-Protein kinase Chk1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O14757
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表达区间
1-476aa
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氨基酸序列
MAVPFVEDWD LVQTLGEGAY GEVQLAVNRV TEEAVAVKIV DMKRAVDCPE NIKKEICINK MLNHENVVKF YGHRREGNIQ YLFLEYCSGG ELFDRIEPDI GMPEPDAQRF FHQLMAGVVY LHGIGITHRD IKPENLLLDE RDNLKISDFG LATVFRYNNR ERLLNKMCGT LPYVAPELLK RREFHAEPVD VWSCGIVLTA MLAGELPWDQ PSDSCQEYSD WKEKKTYLNP WKKIDSAPLA LLHKILVENP SARITIPDIK KDRWYNKPLK KGAKRPRVTS GGVSESPSGF SKHIQSNLDF SPVNSASSEE NVKYSSSQPE PRTGLSLWDT SPSYIDKLVQ GISFSQPTCP DHMLLNSQLL GTPGSSQNPW QRLVKRMTRF FTKLDADKSY QCLKETCEKL GYQWKKSCMN QVTISTTDRR NNKLIFKVNL LEMDDKILVD FRLSKGDGLE FKRHFLKIKG KLIDIVSSQK IWLPAT
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CHEK1, a key protein kinase in the cell cycle checkpoint pathways, plays a critical role in maintaining genomic stability by responding to DNA damage and ensuring proper cell division. This enzyme, part of the ATR (ATM and Rad3-related) signaling cascade, is activated in response to various cellular stresses, including DNA replication stress and double-strand breaks. Mutations or dysregulation of CHEK1 have been implicated in various cancers, making it a potential target for therapeutic interventions. Researchers have focused on the recombinant production of CHEK1 to study its biological functions, regulatory mechanisms, and interaction with other proteins. By using techniques like bacterial expression systems and eukaryotic cell lines, scientists aim to generate functional CHEK1 to investigate its role in checkpoint signaling and cell cycle control. Understanding CHEK1's structure and function through recombinant protein studies may lead to novel insights into cancer biology and the development of new cancer therapies that exploit the vulnerabilities of tumor cells with compromised checkpoint responses. Consequently, the research on CHEK1 not only enhances our understanding of fundamental cellular processes but also contributes to the advancement of targeted cancer treatments.
