Analytical Data
-
Gene name
SIX3
- Application
-
Alternative Names
Homeobox protein SIX3; HPE2; Sine oculis homeobox homolog 3; SIX homeobox 3; Six3; SIX3_HUMAN
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
O95343
-
Expression Region
1-332 aa
-
AA Sequence
MVFRSPLDLY SSHFLLPNFA DSHHRSILLA SSGGGNGAGG GGGAGGGSGG GNGAGGGGAG GAGGGGGGGS RAPPEELSMF QLPTLNFSPE QVASVCETLE ETGDIERLGR FLWSLPVAPG ACEAINKHES ILRARAVVAF HTGNFRDLYH ILENHKFTKE SHGKLQAMWL EAHYQEAEKL RGRPLGPVDK YRVRKKFPLP RTIWDGEQKT HCFKERTRSL LREWYLQDPY PNPSKKRELA QATGLTPTQV GNWFKNRRQR DRAAAAKNRL QHQAIGPSGM RSLAEPGCPT HGSAESPSTA ASPTTSVSSL TERADTGTSI LSVTSSDSEC DV
-
Molecular Weight
35.4 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SIX3 is a member of the SIX homeobox gene family, which plays a crucial role in early embryonic development and the formation of various organ systems, particularly the eyes and brain. Research on SIX3 has gained significant attention due to its involvement in key developmental processes, including the regulation of neural and ocular development. Mutations or dysregulation of SIX3 have been implicated in several congenital disorders, most notably holoprosencephaly, a condition characterized by incomplete separation of the forebrain. The study of SIX3 as a recombinant protein allows for a deeper understanding of its functional properties and interactions with other molecular pathways. By exploring the mechanisms through which SIX3 influences gene expression and cellular behavior, researchers aim to uncover potential therapeutic targets for developmental disorders. Furthermore, its conserved nature across species makes SIX3 a valuable model for investigating evolutionary aspects of developmental biology. Overall, the investigation of SIX3 recombinant protein contributes to our understanding of fundamental developmental processes and holds promise for the development of targeted interventions in congenital anomalies associated with its dysfunction.
