Analytical Data
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Gene name
SHPRH
- Application
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Alternative Names
bA545I5.2; E3 ubiquitin-protein ligase SHPRH; FLJ27258; FLJ37625; FLJ45012; FLJ90837; histone-linker; KIAA2023; MGC134886; PHD and RING finger domain-containing helicase; Shprh; SHPRH_HUMAN; SNF2; SNF2 histone linker PHD RING helicase
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q149N8
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Expression Region
1-522 aa
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AA Sequence
MSPQKRVKNVQAQNRTSQGSSSFQTTLSAWKVKQDPSNSKNISKHGQNNPVGDYEHADDQAEEDALQMAVGYFEKGPIKASQNKDKTLEKHLKTVENVAWKNGLASEEIDILLNIALSGKFGNAVNTRILKCMIPATVISEDSVVKAVSWLCVGKCSGSTKVLFYRWLVAMFDFIDRKEQINLLYGFFFASLQDDALCPYVCHLLYLLTKKENVKPFRVRKLLDLQAKMGMQPHLQALLSLYKFFAPALISVSLPVRKKIYFKNSENLWKTALLAVKQRNRGPSPEPLKLMLGPANVRPLKRKWNSLSVIPVLNSSSYTKECGKKEMSLSDCLNRSGSFPLEQLQSFPQLLQNIHCLELPSQMGSVLNNSLLLHYINCVRDEPVLLRFYYWLSQTLQEECIWYKVNNYEHGKEFTNFLDTIIRAECFLQEGFYSCEAFLYKSLPLWDGLCCRSQFLQLVSWIPFSSFSEVKPLLFDHLAQLFFTSTIYFKCSVLQSLKELLQNWLLWLSMDIHMKPVTNSPL
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Molecular Weight
86.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of SHPRH (SNF2/Historic-Related Protein) recombinant proteins has gained significant attention due to its essential role in DNA repair and genome stability. SHPRH is a member of the SNF2 helicase family and is involved in maintaining cellular homeostasis by promoting the repair of DNA lesions, especially those arising from replication stress and mutagenesis. Deficiencies in SHPRH function have been implicated in various diseases, including cancer, highlighting its potential as a therapeutic target. Researchers focus on the structural and functional characterization of SHPRH recombinant proteins to elucidate their mechanisms in DNA damage recognition and repair pathways. These studies typically involve the expression and purification of SHPRH proteins in heterologous systems, followed by biochemical assays to assess their enzymatic activities, interaction with other repair factors, and impact on cellular DNA repair processes. Understanding the dynamics and regulation of SHPRH can provide insights into its contributions to genomic stability and cancer development, ultimately aiding in the discovery of novel strategies for cancer therapies and preventive measures against genomic instability.
