Analytical Data
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基因名
Btk
- Application
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别名
Btk；AGMX1；ATK；BPK；Tyrosine-Protein kinase BTK
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q06187
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表达区间
1-659aa
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氨基酸序列
MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRG SKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPY PFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDG QYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKK PLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYFILEESNLP WWRARDKNGQEGYIPSNYVTEAEDSIEMYEWYSKHMTRSQAEQLLKQEGK EGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAEKH LFSTIPELINYHQHNSAGLISRLKYPVSQQNKNAPSTAGLGYGSWEIDPK DLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIKEGSMSEDEFIEEAKVMM NLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLE MCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDE YTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYER FTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSN ILDVMDEES
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分子量
78 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Bruton's tyrosine kinase (Btk) is a critical enzyme involved in the signaling pathways of B cells, playing a vital role in immune response and development. Its dysregulation has been implicated in various diseases, particularly in certain types of B cell malignancies and immunodeficiencies. Researchers have been increasingly focused on characterizing Btk due to its potential as a therapeutic target in treating these conditions, especially with the advent of Btk inhibitors in clinical use. The study of recombinant Btk proteins has become essential for understanding the enzyme's structure-function relationships and the downstream signaling mechanisms it mediates. The ability to produce recombinant Btk allows for the examination of its enzymatic activity, interaction with other proteins, and response to potential inhibitors in a controlled laboratory setting. Furthermore, understanding the nuances of Btk function can contribute to the development of more selective therapeutic strategies, aiming to minimize side effects while effectively targeting disease mechanisms. Overall, the investigation of recombinant Btk proteins represents a significant intersection of biochemistry, immunology, and drug development, with the promise of advancing both basic scientific knowledge and clinical applications in managing B cell-related disorders.
